Email this article COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFICATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REALTIME PCR)
- Authors: Panferova Y.A.1, Freilikhman O.A.1, Tokarevich N.K.1, Karpenko S.F.2, Galimzyanov K.M.2
-
Affiliations:
- Pasteur Research Institute of Epidemiology and Microbiology
- Astrakhan State Medical University
- Issue: Vol 93, No 3 (2016)
- Pages: 70-74
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/52
- DOI: https://doi.org/10.36233/0372-9311-2016-3-70-74
- ID: 52
Cite item
Full Text
Abstract
Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). Conclusion. This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.
About the authors
Yu. A. Panferova
Pasteur Research Institute of Epidemiology and Microbiology
Author for correspondence.
Russian Federation
O. A. Freilikhman
Pasteur Research Institute of Epidemiology and MicrobiologyRussian Federation
N. K. Tokarevich
Pasteur Research Institute of Epidemiology and MicrobiologyRussian Federation
S. F. Karpenko
Astrakhan State Medical UniversityRussian Federation
Kh. M. Galimzyanov
Astrakhan State Medical UniversityRussian Federation
References
- Бармин А.Н., Колчин Е.А., Шуваев Н.С., Дмитриева М.В. Анализ проявления природно-очаговых заболеваний на территории Астраханской обл. Естес. науки. 2012, 3 (40): 44-50.
- Садретдинов Р.А, Галимзянов Х.М. Гемодинамические типы микроциркуляции у больных инфекционными лихорадками. Фундамент, исслед. 2010, 7: 63-66.
- Фрейлихман О.А., Панфёрова Ю.А., Токаревич Н.К. Совершенствование метода детекции Coxiella burnetii в биологическом материале на основе амплификации гена groEL. Журн. микробиол. 2010, 4: 71-74.
- Arricau-Bouvery N., HauckY., BejaouiA. etal. Molecular characterisation of Coxiella burnetii isolates by infrequent restriction site-PC Rand MLVA typing. BMC Microbiology. 2006,6 (38). doi: 10.1186/1471-2180-6-38.
- Bielawska-Drozd A., Cieslik P., Mirski T. et al. Q fever - selected issues. Ann. Agric. Environmental Med. 2013, 20 (2): 222-232.
- Freylikhman O., Tokarevich N., Suvorov A. et al. persistence in three generation of mice after application of live attenuated human M-44 vaccine against Q fever. Ann. N.Y. Acad. Sci. 2003, 990: 496-499.
- Jaton K., Peter O., Raoult D. et al. Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period. New Microbes New Infect. 2013, 1 (1): 6-12. doi: 10/1002/2052-2975.8.
- MarrieT., Raoult D. Qfever-a review and issues for the next century. Int. J. Antimicrob. Agents. 1997, 8 (3): 145-161. doi: 10.1016/S0924-8579(96)00369-X.
- Maurin M., Raoult D. Q fever. Clin. Microbiol. Rev. 1999, 12 (4): 518-553.
- Raele D., Garofolo G., Galante D. et al. Molecular detection of Coxiella burnetii using an alternative loop-mediated isothermal amplification assay. Veterinaria Italiana. 2015, 51 (1): 73-78. doi: 10.12834/VetIt.3041168.4.
- Tilburg J., Melchers W., Pettersson A. et al. Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii in serum. J. Clin. Microbiol. 2010, 48 (11): 3924-3927. doi: 10.1128/JCM.01006-10.
- van Schaik E., Chen C., Mertens K. et al. Molecular pathogenesis of obligate intracellular bacterium. Nat. Rev. Microbiol. 2013, 11 (8): 561-573. doi: 10.1038/nrmicro3049.
Supplementary files
