Email this article COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFICATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REALTIME PCR)
- Authors: Panferova Y.A.1, Freilikhman O.A.1, Tokarevich N.K.1, Karpenko S.F.2, Galimzyanov K.M.2
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Affiliations:
- Pasteur Research Institute of Epidemiology and Microbiology
- Astrakhan State Medical University
- Issue: Vol 93, No 3 (2016)
- Pages: 70-74
- Section: ORIGINAL RESEARCHES
- Submitted: 10.04.2019
- Published: 28.06.2016
- URL: https://microbiol.crie.ru/jour/article/view/52
- DOI: https://doi.org/10.36233/0372-9311-2016-3-70-74
- ID: 52
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Full Text
Abstract
Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). Conclusion. This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.
About the authors
Yu. A. Panferova
Pasteur Research Institute of Epidemiology and Microbiology
Author for correspondence.
Email: noemail@neicon.ru
Россия
O. A. Freilikhman
Pasteur Research Institute of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
N. K. Tokarevich
Pasteur Research Institute of Epidemiology and Microbiology
Email: noemail@neicon.ru
Россия
S. F. Karpenko
Astrakhan State Medical University
Email: noemail@neicon.ru
Россия
Kh. M. Galimzyanov
Astrakhan State Medical University
Email: noemail@neicon.ru
Россия
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