IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspCgar+afz OF BORRELIA GARINII AND B. AFZELIIISOLATES

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Abstract

Aim. Comparative study of antigenic properties of recombinant proteins OspCgar and OspCafz and recombinant chimeric polypeptide OspCgar+afZ, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia. Materials and methods. Recombinant chimeric polypeptide OspCgar+afz and recombinant mature proteins OspCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells, purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients. Results. A difference in sensitivity of determination by EIA method of specific IgM and IgG against borreliae in blood sera of LB patients with localized stage of the disease during use of OspCgar, OspCafz and OspCgar+afZ chimera as antigens was shown. Chimeric antigen OspCgar+afz was established to show higher antigenic activity compared with each of the OspCgar or OspCafZ antigens separately. Conclusion. The results of the study allow to examine the recombinant chimeric polypeptide OspCgar+afz as a possible component during creation of test-systems for serodiagnostics of LB on the territory of West Siberia.

About the authors

VS. .. Karavaev

Research Institute of Biochemistry

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

E. S. Oleinikova

Research Institute of Biochemistry; State Scientific Centre of Virology and Biotechnology «Vector»

Email: noemail@neicon.ru
Russian Federation

M. Sh. Azaev

State Scientific Centre of Virology and Biotechnology «Vector»

Email: noemail@neicon.ru
Russian Federation

A. B. Beklemishev'

Research Institute of Biochemistry

Email: noemail@neicon.ru
Russian Federation

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