Email this article INFLUENCE OF ANTIMICROBIAL PEPTIDES OF HUMAN THROMBOCYTES ON STAPHYLOCOCCUS AUREUS BIOFILM FORMATION
- Authors: Zhurlov OS1, Perunova NB1, Ivanova EV1, Egorova OS1
-
Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 66-70
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2012
- URL: https://microbiol.crie.ru/jour/article/view/13728
- ID: 13728
Cite item
Full Text
Abstract
Aim. Study the influence of platelet low molecular weight protein on S. aureus biofilm formation.
Materials and methods. 20 clinical isolates of coagulase-positive staphylococci (S. aureus)
served as material. Preparation containing a mixture of antimicrobial peptides (fractions with
molecular weight 60 - 70 kDa, 20 - 25 kDa and 5 - 10 kDa) obtained from human thrombocyte
donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the
degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system
liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney
criteria. Results. Platelet low molecular weight proteins (PLB) at concentration of 2 g/ml reduced
biofilm formation of S. aureus by 48.79.8% from initial values, increased hydrophobicity of
plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing
platelet low molecular weight proteins was noted at concentration of 50 g/ml. Conclusion. The
data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open
a perspective of further studies of PLB as a preparation suitable for control of biofilm of persistent
microorganisms.
Materials and methods. 20 clinical isolates of coagulase-positive staphylococci (S. aureus)
served as material. Preparation containing a mixture of antimicrobial peptides (fractions with
molecular weight 60 - 70 kDa, 20 - 25 kDa and 5 - 10 kDa) obtained from human thrombocyte
donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the
degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system
liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney
criteria. Results. Platelet low molecular weight proteins (PLB) at concentration of 2 g/ml reduced
biofilm formation of S. aureus by 48.79.8% from initial values, increased hydrophobicity of
plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing
platelet low molecular weight proteins was noted at concentration of 50 g/ml. Conclusion. The
data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open
a perspective of further studies of PLB as a preparation suitable for control of biofilm of persistent
microorganisms.
References
- Брудастов Ю.А., Гриценко В.А., Журлов О.С., Чертков К.Л. Характеристика гидрофобных свойств бактерий при их взаимодействии с сывороткой крови. Журн. микробиол. 1997, 4: 73-77.
- Бухарин О.В., Сулейманов К.Г. Свойства и функции тромбоцитарного катионного белка. Успехи современной биологии. 1998, 2: 194-204.
- Бухарин О.В. Персистенция патогенных бактерий. М., Медицина. 1999.
- Бухарин О.В., Черешнев В.А., Сулейман ов К.Г. Антимикробный белок тромбоцитов. Екатеринбург, УрО РАН, 2000.
- Бухарин О.В., Лобакова Е.С., Перунова Н.Б. и др. Симбиоз и его роль в инфекции. Екатеринбург. УрО РАН, 2011.
- Иванов Ю.Б., Черкасов С.В., Бухарин О.В. Антимикробное средство и фармацевтическая композиция, содержащая эффективное количество антимикробного средства. Патент РФ на изобретение № 2278675, Бюл. 18, 27.06.2006.
- Лакин Г.Ф. Биометрия. М., Высшая школа, 1990.
- Панькова Н.В., Полюдова Т.В., Лемкина Л.М., Коробов В.П. Гидрофобные и донорно-акцепторные свойства поверхности клеток стафилококков, чувствительных и устойчивых к варнерину. Микробиология. 2011, 4: 568-570.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72: 248-254.
- Casterton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: a common cause of persistent infections. Science. 1999, 284: 1318-1322.
- Lewis K. Persister cells and the riddle of biofilm survival. Biochemistry. 2005, 2: 267-274.
- O'Toole G.F., Kaplan H.B., Kolter R. Biofilm formation as microbial development. Ann. Rev. Microbiol. 2000, 54: 49-79.
- Gross M., Cramton S.E. et al. Key role of teichoic acid net charge in Staphylococcus aureus colonization of artificial surfaces. Infect. Immun. 2001, 5: 3423-3426.
Supplementary files
