Email this article INFLUENCE OF ANTIMICROBIAL PEPTIDES OF HUMAN THROMBOCYTES ON STAPHYLOCOCCUS AUREUS BIOFILM FORMATION
- Authors: Zhurlov OS1, Perunova NB1, Ivanova EV1, Egorova OS1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 66-70
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13728
- ID: 13728
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Abstract
Aim. Study the influence of platelet low molecular weight protein on S. aureus biofilm formation.
Materials and methods. 20 clinical isolates of coagulase-positive staphylococci (S. aureus)
served as material. Preparation containing a mixture of antimicrobial peptides (fractions with
molecular weight 60 - 70 kDa, 20 - 25 kDa and 5 - 10 kDa) obtained from human thrombocyte
donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the
degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system
liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney
criteria. Results. Platelet low molecular weight proteins (PLB) at concentration of 2 g/ml reduced
biofilm formation of S. aureus by 48.79.8% from initial values, increased hydrophobicity of
plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing
platelet low molecular weight proteins was noted at concentration of 50 g/ml. Conclusion. The
data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open
a perspective of further studies of PLB as a preparation suitable for control of biofilm of persistent
microorganisms.
Materials and methods. 20 clinical isolates of coagulase-positive staphylococci (S. aureus)
served as material. Preparation containing a mixture of antimicrobial peptides (fractions with
molecular weight 60 - 70 kDa, 20 - 25 kDa and 5 - 10 kDa) obtained from human thrombocyte
donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the
degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system
liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney
criteria. Results. Platelet low molecular weight proteins (PLB) at concentration of 2 g/ml reduced
biofilm formation of S. aureus by 48.79.8% from initial values, increased hydrophobicity of
plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing
platelet low molecular weight proteins was noted at concentration of 50 g/ml. Conclusion. The
data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open
a perspective of further studies of PLB as a preparation suitable for control of biofilm of persistent
microorganisms.
References
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