TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA

Yu. V. Ostankova; Останкова Ю. В.; A. V. Semenov; Семенов А. В.; N. A. Stoyanova; Стоянова Н. А.; N. K. Tokarevich; Токаревич Н. К.; N. E. Lyubimova; Любимова Н. Е.; O. A. Petrova; Петрова О. А.; Yu. V. Ananina; Ананьина Ю. В.; E. M. Petrov; Петров Е. М.

doi:10.36233/0372-9311-2016-1-35-39

TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA

Authors: Ostankova Y.V.¹, Semenov A.V.¹, Stoyanova N.A.¹, Tokarevich N.K.¹, Lyubimova N.E.¹, Petrova O.A.¹, Ananina Y.V.², Petrov E.M.²
Affiliations:
1. Pasteur Research Institute of Epidemiology and Microbiology
2. Gamaleya Federal Research Centre of Epidemiology and Microbiology
Issue: Vol 93, No 1 (2016)
Pages: 35-39
Section: ORIGINAL RESEARCHES
URL: https://microbiol.crie.ru/jour/article/view/7
DOI: https://doi.org/10.36233/0372-9311-2016-1-35-39
ID: 7

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Abstract

Aim. Comparative typing of Leptospira spp. strain collection based on analysis of 16S RNA fragment. Materials and methods. 2 pairs of primers were used for PCR, that jointly flank 1423 b.p. sized fragment. Sequences of Leptospira spp. strain 16S rRNA, presented in the international database, were used for phylogenetic analysis. Results. A high similarity, including interspecies, of the 16S fragment in Leptospira spp. strains was shown independently of the source, serovar and serogroup. Heterogeneity ofthe primary matrix, spontaneous mutations of hotspots and erroneous nucleotide couplings, characteristic for 16S sequence of pathogenic Leptospira spp. strains, are discussed. Molecular-genetic characteristic of certain reference Leptospira spp. strains by 16S sequence is obtained. Conclusion. Results of the studies give evidence on expedience of introduction into clinical practice of identification of Leptospira spp. by 16S sequence directly from the clinical material, that would allow to significantly reduce identification time, dismiss complex type-specific sera and other labor-intensive methods.

Keywords

16S РНК секвенирование, eptospirosis, zoo anthroponoses, 16S RNA sequencing

References

Ананьина Ю.В. Лептоспирозы в Российской Федерации: современные особенности эпидемического проявления природных и техногенных очагов. Ветеринарная патология. 2004, 4: 54-57.
Стоянова Н.А., Токаревич Н.К., Ваганова А.Н. Лептоспирозы: пособие для врачей. Санкт-Петербург, 2010.
Chappel R.J., Goris M., Palmer M.F. Impact of proficiency testing on results of the microscopic agglutination test for diagnosis of leptospirosis. J. Clin. Microbiol. 2004, 42: 54845488.
Cerqueira G.M., McBride A.J.A., Queiroz A. et al. Monitoring leptospira strain collections: The need for quality control. Am. J. Trop. Med. Hyg. 2010, 82 (1): 83-87.
Faine S., Adler B., Bolin C. Leptospira and Leptospirosis. Melbourn, Australia, 1999.
Morey R.E., Galloway R.L., Bragg S.L. Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. J. Clin. Microbiol. 2006, 44: 3510-3516.
Postic D., Riquelme-Sertour N., Merien F. Interest of partial 16S rDNA gene sequences to resolve heterogeneities between Leptospira collections: application to L. meyeri. Res. Microbiol. 2000, 151 (5): 333-341.
Sagunan A. Risk factor associated with leptospirosis during an outbreak in Middle Andaman, India. Indian J. Med. Res. 2009, 130 (1): 114-116.

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TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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