MODELLING OF HEPATITIS E IN MINI-PIGS

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Abstract

Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in 2013. The virus was administered as a 10% clarified feces extract (800 pi). Blood (weekly) and feces (daily) were sampled from the animals for 49 days. Anti-HEV IgG were determined in sera samples using DS- ElA-ANTI-HEV-G (Diagnostic Systems) system, HEV RNA in samples of feces extracts and blood sera - RT-PCR. Results. Wiesenauer minipigs were sensitive to HEV genotype 3 infection, infection developed in all the animals after administration of the virus, that was accompanied by the presence of HEV RNA in feces for 2 weeks and seroconversion by anti-HEV. Comparative analysis of virological characteristics of experimental H EV-infection in mini- and standard pigs has shown, that the duration of the infection in mini-pigs is shorter, and seroconversion by anti-HEV occurs earlier. Conclusion. Sensitivity to HEV genotype 3 and lower weight make mini-pigs a comfortable alternative to standard swine and primates for modelling HEV infection in vivo.

About the authors

S. A. Gulyaev

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Author for correspondence.
Russian Federation

I. A. Potemkin

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

V. S. Kichatova

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

A. A. Karlsen

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

O. V. Isaeva

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

T. V. Guiyaeva

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Russian Federation

M. A. Vannus

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Russian Federation

I. V. Gordeichuk

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Russian Federation

K. K. Kyuregyan

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

M. I. Mikhailov

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Russian Federation

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Copyright (c) 2017 Gulyaev S.A., Potemkin I.A., Kichatova V.S., Karlsen A.A., Isaeva O.V., Guiyaeva T.V., Vannus M.A., Gordeichuk I.V., Kyuregyan K.K., Mikhailov M.I.

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