MODELLING OF HEPATITIS E IN MINI-PIGS

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Abstract

Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in 2013. The virus was administered as a 10% clarified feces extract (800 pi). Blood (weekly) and feces (daily) were sampled from the animals for 49 days. Anti-HEV IgG were determined in sera samples using DS- ElA-ANTI-HEV-G (Diagnostic Systems) system, HEV RNA in samples of feces extracts and blood sera - RT-PCR. Results. Wiesenauer minipigs were sensitive to HEV genotype 3 infection, infection developed in all the animals after administration of the virus, that was accompanied by the presence of HEV RNA in feces for 2 weeks and seroconversion by anti-HEV. Comparative analysis of virological characteristics of experimental H EV-infection in mini- and standard pigs has shown, that the duration of the infection in mini-pigs is shorter, and seroconversion by anti-HEV occurs earlier. Conclusion. Sensitivity to HEV genotype 3 and lower weight make mini-pigs a comfortable alternative to standard swine and primates for modelling HEV infection in vivo.

About the authors

S. A. Gulyaev

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

I. A. Potemkin

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

V. S. Kichatova

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

A. A. Karlsen

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

O. V. Isaeva

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

T. V. Guiyaeva

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Email: noemail@neicon.ru
Russian Federation

M. A. Vannus

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Email: noemail@neicon.ru
Russian Federation

I. V. Gordeichuk

Chumakov Institute of Poliomyelitis and Viral Encephalitis

Email: noemail@neicon.ru
Russian Federation

K. K. Kyuregyan

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

M. I. Mikhailov

Russian Medical Academy of Continuous Professional Education, Mechnikov Research Institute of Vaccines and Sera

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2017 Gulyaev S.A., Potemkin I.A., Kichatova V.S., Karlsen A.A., Isaeva O.V., Guiyaeva T.V., Vannus M.A., Gordeichuk I.V., Kyuregyan K.K., Mikhailov M.I.

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