INFLUENCE OF STAPHYLOCOCCUS VACCINE ON FUNCTIONAL ACTIVITY OF ANTIGEN-PRESENTING CELLS

Aim. Study the influence of staphylococcus vaccine on functional activity of antigen-presenting cells. Materials and methods. Mice intraperitoneally received 500 ^g of «Staphylovac» vaccine. Phagocytic activity of peritoneal macrophages against Staphylococcus aureus 1991 was determined in animals at various time intervals. Phagocytic index (PI) and phagocytic number (PN) in smears made at 30 and 60 minutes of incubation were calculated. Dendritic cells (DC) were obtained from bone marrow precursors during cultivation with 20 ng/ml GM-CSF and 20 ng/ml IL-4 (BioSource International Inc., Belgium). At day 6 of incubation staphylococcus vaccine (50 ^g/ml) was added to immature cells for induction of DC maturation. DC phenotype evaluation was carried out by flow cytometry using monoclonal antibodies against cell antigens (Beckman Culter, USA). Results. PI at 30 and 60 minutes of incubation increased by 0.12 - 1.4 times and 1.11 - 1.52 times, respectively, compared with control. PN at 30 minutes of incubation of cells with microbial suspension increased from 8.6 to 11.4% against 5.9% in control, at 60 minutes of incubation - from 7.7 to 8.1% against 5.1% in control. In DC culture during their incubation with the vaccine, content of cells with expression of intercellular adhesion marker CD38, antigen presenting marker MHCII and DC terminal differentiation marker CD83 increased. Expression of CD34 and CD14 was also noted, that may give evidence on partial direction of cell differentiation to macrophages. Conclusion. «Staphylovac» vaccine during intraperitoneal administration to mice had activating influence on functional activity of antigen-presenting cells and peritoneal macrophages.

дендритные клетки, «Стафиловак», иммунофенотип, фагоцитоз