GENOTYPING OF PARVOVIRUS B19 ISOLATES CIRCULATING IN NORTHWESTERN FEDERAL DISTRICT OF RUSSIA


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Abstract

Im. Genotyping and phylogenetic analysis of parvovirus B19 isolates isolated on the territories of Northwestern Federal District (NWFD) of Russia. Materials and methods. 61 blood sera and 30 oropharyngeal lavages obtained from patients with maculopapular rash from various territories of NWFD were studied for the presence of parvovirus B19 DNA (PVB19). DNA isolation and amplification was carried out by standard techniques. DNA segment including fragment of non-structural gene NS1 and region of structural gene VP1 (NS1-VP1u, 994 nucleotides) was sequenced, original sequences of oligonucleotide primers were selected for this purpose. Phylogenetic analysis was carried out online on the website http://www.phylogeny.fr. Data for tree construction was obtained from GenBank. Results. PVB19 DNA was detected in 45% ofsamples. PVB19 genome segment was sequenced in 8 samples. All the PVB19 isolates belong to a single cluster of 1A genotype. Isolate 57.12 from Komi Republic is similar to ISR-G strain isolated from Israel. Conclusion. Phylogenetic analysis showed a high degree of genetic similarity between PVB19 isolates circulating on the territories of NWFD, their membership in the most widespread genotype in the world. Local and import cases of parvovirus infection (PVI) were identified. The authors make a conclusion on the necessity to include PVI into the system of rubella and measles control.

About the authors

I. N Lavrentieva

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

A. Yu Antipova

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

A. V Semenov

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

M. A Bichurina

Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia

References

  1. Антипова А.Ю., Лаврентьева И.Н., Бичурина М.А. и др. Распространение парвовирусной инфекции в Северо-Западном федеральном округе России. Журнал инфектологии. 2011, 3(4): 44-48.
  2. Васильев В.В., Мурина Е.А., Сидоренко С.В. и др. Парвовирусная ^19V инфекция у беременных и детей раннего возраста. Журнал инфектологии. 2011, 3(4):26-33.
  3. Взятие, транспортировка, хранение клинического материала для ПЦР-диагностикии. Методическое пособие. Справочно-информационное издание ИнтерЛабСервис. М., Роспотребнадзор, ЦНИИЭ, 2010.
  4. Лялина Л.В., Терентьева Ж.В., Бичурина М.А. и др. Влияние двукратной иммунизации на заболеваемость корью, эпидемическим паротитом и краснухой в Северо-Западном федеральном округе России. Инфекция и иммунитет. 2012, 2 (4): 753-756.
  5. Маниатис Т., Фрич Э., Сэмбрук Дж. Методы генетической инженерии. Молекулярное клонирование. М., Мир, 1984.
  6. Anisimova M., Gascuel O. Approximate likelihood ratio test for branchs: A fast, accurate and powerful alternative. Syst. Biol. 2006, 55 (4): 539-552.
  7. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 2000, 17 (4): 540-552.
  8. Chevenet F., Brun C., Banuls A.L. et al. TreeDyn: towards dynamic graphics and annotations for analyses of trees. BMC Bioinformatics. 2006, 10 (7): 439.
  9. Christensen J., Cotmore S.F., Tattersall P. A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication. J. Virol. 1997, 71: 1405-1416.
  10. Corcoran C., Hardie D., Yeats J. et al. Genetic variants of human parvovirus B19 in South Africa: Cocirculation of three genotypes and identification of a novel subtype of genotype 1. J. Clin. Microbiol. 2010, 48 (1):137-142.
  11. Dereeper A., Audic S., Claverie J.M. et al. BLAST-EXPLORER helps you building datasets for phylogenetic analysis. BMC Evol. Biol. 2010, 12 (10): 8.
  12. Dereeper A., Guignon V., Blanc G. et al. Phylogeny.fr: robust phylogenetic analysis for the nonspecialist. Nucleic Acids Res. 2008, 36 (Web Server issue): W 465-469.
  13. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32 (5): 1792-1797.
  14. Guindon S., Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 2003, 52 (5): 696-704.
  15. Hemauer A., von Poblotzki A., Gigler A. et al. Sequence variability among different parvovirus B19 isolates. J. General Virology. 1996, 77: 1782-1785.
  16. Htibschen J.M., Mihneva Z., Mentis A.F. et al. Phylogenetic analysis of human parvovirus B19 sequences from eleven different countries confirms the predominance of genotype 1 and suggests the spread of genotype 3b. J. Clin. Microbiol. 2009, 47 (11): 3735-3738.
  17. Marchler-Bauer A., Lu Sh., Anderson J.B. et al. CDD: a conserved domain database for the functional annotation of proteins. Nucleic Acids Res. 2011, 39 (D): 225-229.
  18. Schneider B., Hone A., Tolba R.H. et al. Simultaneous persistence of multiple genome variants of human p[arvovirus B19. J. Genetic Virology. 2008, 89: 164-176.
  19. Servant A., Laperche S., Lallemand F. et al. Genetic diversity within human erythroviruses: identification of three genotypes. J. Virology. 2002, 76 (18): 9124-9134.
  20. Toan N.L., Duechting A., Kremsner P.G. et al. Phylogenetic analysis of human parvovirus B19,indicating two subgroups of genotype 1 in Vietnamese patients. J. General Virology. 2006, 87: 2941-2949.
  21. Yermalovich M.A., Htibschen J.M., Semeiko G.V et al. Human parvovirus B19 surveillance in patients with rash and fever from Belarus. J. Med. Virol. 2012, 84 (6): 973-978.

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Copyright (c) 2013 Lavrentieva I.N., Antipova A.Y., Semenov A.V., Bichurina M.A.

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