FEATURES OF PROLIFERATIVE RESPONSE OF T-CELLS ON HEPATITIS C VIRUS ANTIGENS IN HEALTHY INDIVIDUALS CONTACTING WITH THIS VIRUS


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Abstract

Aim. Evaluation of CD3+ and CD3-/CD56+ proliferative response on hepatitis C virus antigens in healthy medical workers. Materials and methods. The study included 15 medical workers with length of service of 2 and more years without common risk factors (blood and blood products transfusion, abdominal operations, invasive procedures, use of intravenous drugs). Control group consisted of 9 healthy individuals without risk factors. Peripheral mononuclears were isolated from blood and then incubated 72 hours in the presence of mitogen/PHA, Core+NS4 1b genotype HCV, NS4 HCV 2a+3a genotypes or medium. Proliferative activity was registered by the presence of cell division marker ki67 by using FACS. Results. The initial immunogram of medical workers differed from the control group by a significantly lower quantity of CD3+ lymphocytes, in particular CD3+/CD8 + population. Incubation with PHA resulted in a decrease of quantity ofCD3+/ ki67+, CD4+/ki67+ and CD3-/CD56+/ki67+ in the medical workers group. Cultivation with HCV antigens resulted in a significant decrease of Treg (CD3+/CD25high/FoxP3+) and activated T-lymphocytes population in the case of stimulation by Core and NS4 1b genotype antigens. Analysis of cell response on virus antigens based on proliferative activity index detected significant differences only for CD8+/ki67+. Stimulation by Core and NS4 1b genotype antigens resulted in an increase of quantity of these cells whereas in the case of NS4 2a+3a genotypes their decrease was observed. Conclusion. The described changes may reflect exhaustion of cell reactivity due to extra antigen load in the group of medical workers, while differentiated immunologic shifts on hepatitis C viruses of various genotypes are noted.

About the authors

N. V Fedoseeva

Moscow State Medical-Stomatological University, Russia

E. V Shmeleva

Moscow State Medical-Stomatological University, Russia

O. S Kapitonova

Moscow State Medical-Stomatological University, Russia

I. P Balmasova

Moscow State Medical-Stomatological University, Russia

M. M Gultyaev

Moscow State Medical-Stomatological University, Russia

O. O Znoiko

Moscow State Medical-Stomatological University, Russia

N. D Yuschyk

Moscow State Medical-Stomatological University, Russia

References

  1. Al-Sherbiny M., Osman A., Mohamed N. et al. Exposure to hepatitis C virus induces cellular immune responses without detectable viremia or seroconversion. Am. J. Trop. Med. Hyg. 2005, 73 (1): 44-49.
  2. Bes M., Esteban J.I., Casamitjana N. et al. Hepatitis C virus (HCV)-specific T-cell responses among recombinant immunoblot assay-3-indeterminate blood donors: a confirmatory evidence of HCV exposure. Transfusion. 2009, 49 (7): 1296-3105.
  3. Edwards V.C., Tarr A.W, Urbanowicz R. A. et al. The role of neutralizing antibodies in hepatitis C virus infection. General Virology. 2012, 93: 1-19.
  4. Kamal S. M. et al. Cellular immune responses in seronegative sexual contacts of acute hepatitis C patients. J. Virol. 2004, 78: 12252-12258.
  5. Koziel M. J., Wong D. K., Dudley D. et al. Hepatitis C virus-specific cytolytic T lymphocyte and T helper cell responses in seronegative persons. J. Infect. Dis. 1997, 176: 859-866.
  6. Neumann-Haefelin C., Blum H.E., Chisari F.V. et al. T cell response in hepatitis C virus infection. Rev. J. Clin. Virol. 2005, 32: 75-85.
  7. Prem H. Thurairajah, Hegazy D., Chokshi S. et al. Hepatitis C virus (HCV)-specific T cell responses in injection drug users with apparent resistance to HCV infection. J. Infect. Dis. 2008, 198: 1749-1755.
  8. Rehermann B. Hepatitis C virus versus innate and adaptive immune responses: a tale of coevolution and coexistence. J. Clin. Invest. 2009, 119 (7): 1745-1754.
  9. Scognamiglio P., Accapezzato D., Casciaro M.A. et al. Presence of effector CD8+ T Cells in hepatitis C virus-exposed healthy seronegative donors. J. Immunology. 1999, 162: 66816689.
  10. Takaki A., Wiese M., Maertens G. et al. Cellular immune responses persist and humoral responses decrease two decades after recovery from a single-source outbreak of hepatitis C. Nature Medicine. 2000, 6: 578-582.
  11. Zeremski M. et al. Hepatitis C virus-specific T-cell immune responses in seronegative injection drug users. J. Viral Hepatitis. 2009, 16: 10-20.

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Copyright (c) 2013 Fedoseeva N.V., Shmeleva E.V., Kapitonova O.S., Balmasova I.P., Gultyaev M.M., Znoiko O.O., Yuschyk N.D.

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