FORMATION OF MICROBIAL BIOFILMS IN CAUSATIVE AGENTS OF ACUTE AND CHRONIC PYELONEPHRITIS

Aim. Study the intensity of formation of microbial biofilms by Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus strains isolated during various forms of pyelonephritis. Materials and methods. 150 clinical isolates of microorganisms isolated from urine of patients with acute and chronic pyelonephritis were included into the study. Determination of intensity of film-formation was carried out by staining of the formed biofilms by crystal violet with consequent extraction of the dye and measurement of its concentration in washout solution. Results. Among causative agents of pyelonephritis P. aeruginosa isolates had the maximum film-forming ability. The intensity of biofilm formation of these isolates was 2-3 time higher than staphylococcus and enterobacteria strains. Strains isolated from patients with chronic pyelonephritis by ability to form biofilms significantly surpassed strains isolated from acute pyelonephritis patients. A higher ability to form microbial biofilms for microorganisms - causative agents of pyelonephritis progressing against the background of urolithiasis was noted. Conclusion. The ability to form biofilms is determined by both causative agent species and character of the infectious process in which this microorganism participates. Intensive formation of biofilms by E. coli, P. aeruginosa, K. pneumoniae, S. aureus clinical isolates may be an important factor of chronization of urinary tract infections.

Pseudomonas aeruginosa, biofilms, enterobacteria, staphylococci, Pseudomonas aeruginosa, acute and chronic pyelonephritis