EFFECT OF STRAIN-PRODUCER AND CULTIVATION MEDIUM ON CROSS ANTIGENIC ACTIVITY OF S TREPTOCOCCUS PNEUMONIAE WATER SOLUBLE ANTIGENS


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Abstract

Aim. Production of water soluble protein-containing antigens from various strains of S. pneumoniae during cultivation in complete and semi-synthetic culture media as well as selection of strains with cross antigenic activity. Materials and methods. S. pneumoniae 3, 6А, 6В, 14, 10А, 18А, 19А, 19F, 23F serotype strains were cultivated in brain-heart broth and semi-synthetic medium with addition of aminopeptide for 24 hours at 37°C for the production of water soluble antigens. The antigens were obtained by a method of triple water extraction from acetone dried microbial cells. Chemical composition of preparations, electrophoresis mobility of protein-containing components of preparations and cross antigenic activity in gel immune diffusion reaction by using rabbit hyperimmune sera were studied. Results. In studies of 10 pneumococcus strains from various serotypes a method of microbial cell inactivation by acetone was selected that allows to produce preparations with high protein content (25.5 — 53.1%). Electrophoretic separation of the preparations revealed difference in the preparations obtained from various pneumococcus strains in the layout of major protein lines in the 8 — 95 kDa range. The most virulent and immunogenic S. pneumoniae strain that during cultivation in semi-synthetic medium was characterized by intraspecies cross antigenic activity and in gel immune diffusion reacted with all the studied sera against 3, 14, 18С, 23F serotype strains was selected. Conclusion. The study resulted in the selection of a technologically simple method of production of pneumococcus antigens with high protein content and showed that only 1 of the studied preparations produced from a virulent strain with poorly expressed S. pneumoniae capsule during cultivation in semi-synthetic medium has the highest cross antigenic activity.

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ВЛИЯНИЕ ШТАММА-ПРОДУЦЕНТА И СРЕДЫ КУЛЬТИВИРОВАНИЯ НА ПЕРЕКРЕСТНУЮ АНТИГЕННУЮ АКТИВНОСТЬ ВОДОРАСТВОРИМЫХ АНТИГЕНОВ STREPTOCOCCUS PNEUMONIAE
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About the authors

E. A Kurbatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

D. S Vorobyev

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. B Egorova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

S. I Elkina

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. G Kalina

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

M. M Tokarskaya

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

A. P Baturo

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. E Romanenko

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

M. E Markova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. V Grischenko

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. N Ovechko

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

Yu. V Volokh

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

S. A Zlygostev

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. A Mikhaylova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

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Copyright (c) 2013 Kurbatova E.A., Vorobyev D.S., Egorova N.B., Elkina S.I., Kalina N.G., Tokarskaya M.M., Baturo A.P., Romanenko E.E., Markova M.E., Grischenko N.V., Ovechko N.N., Volokh Y.V., Zlygostev S.A., Mikhaylova N.A.

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