EPIDEMIOLOGIC DIAGNOSTIC OF NOSOCOMIAL SUPPURATIVE-SEPTIC INFECTIONS OF PSEUDOMONAS ETIOLOGY BASED ON INTRASPECIES TYPING OF CAUSATIVE AGENT

Aim. Scientific justification of optimization of epidemiologic diagnostic of suppurative-septic infection (SSI) caused by Pseudomonas aeruginosa based on comparability of antibiotic sensitivity and β-lactamase production. Materials and methods. Intraspecies typing of 37 P. aeruginosa strains isolated during microbiological monitoring of 106 patients and 131 objects of clinical environment of surgical and obstetrician hospitals by using a complex of phenotypic and molecular-biological methods including determination of sensitivity to antibiotics by serial dilutions method and PCR-diagnostics with determination of TEM, SHV, CTX, OXA, MBL, VIM genes was performed. Results. P. aeruginosa strains combined into groups by isolation location during studies turned out to be heterogeneous by sensitivity to antibiotics and β-lactamase production that al- lowed to form subgroups of strains by focality attribute. Isolates recovered from different SSI foci had significant differences in minimal inhibitory concentration (MIC) reaching 1024 times. MIC parameter within subgroups did not exceed 8 — 16 consequent dilutions. Conclusion. Use of a complex of phenotypic and molecular-biologic methods of causative agent typing including determination of sensitivity to antibiotics by serial dilutions method and evaluation of β-lactamase production allowed to establish a mechanism of development of SSI epidemic process caused by P. aeruginosa , detect origins and reservoirs of infection in hospital, modes and factors of transmission and reach maximum justification of epidemiologic control and prophylaxis measures of localization of foci of nosocomial infections of pseudomonas etiology.

P. aeruginosa, nosocomial infections, sensitivity to antibacterial preparations, molecular- genetic typing, epidemiological diagnostic