ACTIVATION OF LYMPHOCYTES UNDER THE INFLUENCE OF AN INFLUENZA VACCINE COMBINEDWITH A LOW MOLECULAR WEIGHT GERMANIUM ORGANIC COMPOUND

Aim. Confirmation of immunostimulating effect of an original low molecular weight germanium organic
compound (LMW-GOC) during immunization of mice with Vaxigrip vaccine. Materials and methods.
The experiments were carried out in CBA mice divided into 4 groups: control, those that received
Vaxigrip influenza vaccine intraperitoneally, those that received LMW-GOC intraperitoneally and those
that received both preparations at once. Effect of the preparations administered was evaluated by flow
cytofluorometry based on changes of СD3, CD4, CD5, CD8, CD19, CD25, Foxp3, NK1.1, ƒƒ T, MHC
II, TLR2, TLR4, TLR9 expressing cell content in mice spleens. The content of the colored cells was determined
at normal, 24 hours and 7 days after the administration of the preparations. Statistical treatment
of the data was carried out by using Win MD 128 program package. Results. LMW-GOC can enhance the
effect of Vaxigrip vaccine that is expressed by an increase of content in spleen of some lymphocyte subpopulations
24 hours and 7 days after the intraperitoneal administration. In some cases LMW-GOC increases
the content of some lymphocyte subpopulations in mice spleen after administration as a monopreparation,
i.e. without the vaccine. LMW-GOC suppressed stimulating effect of the vaccine on the spleen
content of various lymphocyte subpopulations in none of the observations. Conclusion. By using cytofluorometry
method it is possible to form an understanding of an elevated role of various types of cells in the
development of immune response to the vaccine as well as regarding additional enhancement of this response
during administration of LMW-GOC to mice. The effect of the preparation is manifested for a few
days after its administration. The preparation manifests adjuvant properties and after further studies may
be suggested for use as an adjuvant.

germanium organic compound, TLRs, mice lymphocyte markers, Vaxigrip vaccine