Email this article MODEL OF CHRONIC SALMONELLOSIS: PARAMETERS OF INFECTION AND IMMUNE RESPONSE IN INBRED MICE GENETICALLY VARIABLE IN SUSCEPTIBILITY TO SALMONELLOSIS
- Authors: Nesterenko LN1, Kobets NV1, Balunets DV1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 9-14
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2012
- URL: https://microbiol.crie.ru/jour/article/view/13675
- ID: 13675
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Abstract
Aim. Study parameters of chronic infection and immune response in I/St and A/Sn line mice
in the model of per oral infection of mice with Salmonella enterica serovar Typhimurium. Materials
and methods. Studies were carried out in I/StSnEgYCit (I/St), A/JsnYCit (A/Sn) inbred line mice
as well as their back crossing hybrids [I/StrxF1(I/StxA/Sn)]BC. Mice were infected per os by S.
enterica serovar Тyphimurium strain IE147 at a dose of 2x105 PFU per mice. The number of salmonellae
was determined at days 3, 5 and 7, weeks 3 and 4 after the infection in various organs,
the number of antibody producers - by cell EIA. Pathomorphologic changes in mice spleens were
studied histologically by using hematoxylin and eosin staining. In offspring of back crossing [I/St
x F1(I/St x A/Sn)]ВС1 segregation genetic analysis of sensitivity to salmonella infection trait and
mapping of loci taking part in salmonella infection were carried out. Results. The course of
chronic salmonellosis in susceptible I/St line was characterized by the presence of more pronounced
pathomorphologic changes in spleen and significantly higher microbial load in organs (approximately
by 1000 times) when compared with A/Sn mice. Interlinear differences in susceptibility to
infection correlated with differences in the type of early local and systemic immune response. In
I/St mice a higher level of salmonella specific IgG2a-, IgG1- and IgA forming cells in spleen
compared with A/Sn mice was detected which correlates with a pronounced splenomegaly and
high concentration of salmonellae. On the contrary A/Sn mice demonstrated a higher level of
salmonella specific IgA forming cells in Peyer patches that probably leads to protection of A/Sn
line during per oral infection. Genetic analysis of susceptibility to salmonellosis trait inheritance
showed the presence of its coupling with D9Mit89 locus of chromosome 9 on which previously
Tbs2 locus was mapped that plays a role in the control of tuberculosis infection. Conclusion. There
is a probability of the presence of general mechanisms of genetic control of tuberculosis and salmonella
infections in A/Sn and I/St mice.
in the model of per oral infection of mice with Salmonella enterica serovar Typhimurium. Materials
and methods. Studies were carried out in I/StSnEgYCit (I/St), A/JsnYCit (A/Sn) inbred line mice
as well as their back crossing hybrids [I/StrxF1(I/StxA/Sn)]BC. Mice were infected per os by S.
enterica serovar Тyphimurium strain IE147 at a dose of 2x105 PFU per mice. The number of salmonellae
was determined at days 3, 5 and 7, weeks 3 and 4 after the infection in various organs,
the number of antibody producers - by cell EIA. Pathomorphologic changes in mice spleens were
studied histologically by using hematoxylin and eosin staining. In offspring of back crossing [I/St
x F1(I/St x A/Sn)]ВС1 segregation genetic analysis of sensitivity to salmonella infection trait and
mapping of loci taking part in salmonella infection were carried out. Results. The course of
chronic salmonellosis in susceptible I/St line was characterized by the presence of more pronounced
pathomorphologic changes in spleen and significantly higher microbial load in organs (approximately
by 1000 times) when compared with A/Sn mice. Interlinear differences in susceptibility to
infection correlated with differences in the type of early local and systemic immune response. In
I/St mice a higher level of salmonella specific IgG2a-, IgG1- and IgA forming cells in spleen
compared with A/Sn mice was detected which correlates with a pronounced splenomegaly and
high concentration of salmonellae. On the contrary A/Sn mice demonstrated a higher level of
salmonella specific IgA forming cells in Peyer patches that probably leads to protection of A/Sn
line during per oral infection. Genetic analysis of susceptibility to salmonellosis trait inheritance
showed the presence of its coupling with D9Mit89 locus of chromosome 9 on which previously
Tbs2 locus was mapped that plays a role in the control of tuberculosis infection. Conclusion. There
is a probability of the presence of general mechanisms of genetic control of tuberculosis and salmonella
infections in A/Sn and I/St mice.
References
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