Email this article MODEL OF CHRONIC SALMONELLOSIS: PARAMETERS OF INFECTION AND IMMUNE RESPONSE IN INBRED MICE GENETICALLY VARIABLE IN SUSCEPTIBILITY TO SALMONELLOSIS
- Authors: Nesterenko LN1, Kobets NV1, Balunets DV1
-
Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 9-14
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13675
- ID: 13675
Cite item
Full Text
Abstract
Aim. Study parameters of chronic infection and immune response in I/St and A/Sn line mice
in the model of per oral infection of mice with Salmonella enterica serovar Typhimurium. Materials
and methods. Studies were carried out in I/StSnEgYCit (I/St), A/JsnYCit (A/Sn) inbred line mice
as well as their back crossing hybrids [I/StrxF1(I/StxA/Sn)]BC. Mice were infected per os by S.
enterica serovar Тyphimurium strain IE147 at a dose of 2x105 PFU per mice. The number of salmonellae
was determined at days 3, 5 and 7, weeks 3 and 4 after the infection in various organs,
the number of antibody producers - by cell EIA. Pathomorphologic changes in mice spleens were
studied histologically by using hematoxylin and eosin staining. In offspring of back crossing [I/St
x F1(I/St x A/Sn)]ВС1 segregation genetic analysis of sensitivity to salmonella infection trait and
mapping of loci taking part in salmonella infection were carried out. Results. The course of
chronic salmonellosis in susceptible I/St line was characterized by the presence of more pronounced
pathomorphologic changes in spleen and significantly higher microbial load in organs (approximately
by 1000 times) when compared with A/Sn mice. Interlinear differences in susceptibility to
infection correlated with differences in the type of early local and systemic immune response. In
I/St mice a higher level of salmonella specific IgG2a-, IgG1- and IgA forming cells in spleen
compared with A/Sn mice was detected which correlates with a pronounced splenomegaly and
high concentration of salmonellae. On the contrary A/Sn mice demonstrated a higher level of
salmonella specific IgA forming cells in Peyer patches that probably leads to protection of A/Sn
line during per oral infection. Genetic analysis of susceptibility to salmonellosis trait inheritance
showed the presence of its coupling with D9Mit89 locus of chromosome 9 on which previously
Tbs2 locus was mapped that plays a role in the control of tuberculosis infection. Conclusion. There
is a probability of the presence of general mechanisms of genetic control of tuberculosis and salmonella
infections in A/Sn and I/St mice.
in the model of per oral infection of mice with Salmonella enterica serovar Typhimurium. Materials
and methods. Studies were carried out in I/StSnEgYCit (I/St), A/JsnYCit (A/Sn) inbred line mice
as well as their back crossing hybrids [I/StrxF1(I/StxA/Sn)]BC. Mice were infected per os by S.
enterica serovar Тyphimurium strain IE147 at a dose of 2x105 PFU per mice. The number of salmonellae
was determined at days 3, 5 and 7, weeks 3 and 4 after the infection in various organs,
the number of antibody producers - by cell EIA. Pathomorphologic changes in mice spleens were
studied histologically by using hematoxylin and eosin staining. In offspring of back crossing [I/St
x F1(I/St x A/Sn)]ВС1 segregation genetic analysis of sensitivity to salmonella infection trait and
mapping of loci taking part in salmonella infection were carried out. Results. The course of
chronic salmonellosis in susceptible I/St line was characterized by the presence of more pronounced
pathomorphologic changes in spleen and significantly higher microbial load in organs (approximately
by 1000 times) when compared with A/Sn mice. Interlinear differences in susceptibility to
infection correlated with differences in the type of early local and systemic immune response. In
I/St mice a higher level of salmonella specific IgG2a-, IgG1- and IgA forming cells in spleen
compared with A/Sn mice was detected which correlates with a pronounced splenomegaly and
high concentration of salmonellae. On the contrary A/Sn mice demonstrated a higher level of
salmonella specific IgA forming cells in Peyer patches that probably leads to protection of A/Sn
line during per oral infection. Genetic analysis of susceptibility to salmonellosis trait inheritance
showed the presence of its coupling with D9Mit89 locus of chromosome 9 on which previously
Tbs2 locus was mapped that plays a role in the control of tuberculosis infection. Conclusion. There
is a probability of the presence of general mechanisms of genetic control of tuberculosis and salmonella
infections in A/Sn and I/St mice.
References
- Fortin A., Abel L., Casanova J. L., Gros P. Host genetics of mycobacterial diseases in mice and men: forward genetic studies of BCG-osis and tuberculosis. Ann. Rev. Genomics Hum. Genet. 2007, 8: 163-192.
- Kobets N., Kennedy K., O'Donnell D., Garside P. An investigation of the ability of orally primed and tolerised T cells to help B cells upon mucosal challenge. Immunology. 2004, 112 (4): 550-558.
- Monack D.M., Bouley D.M., Falkow S. Salmonella typhimurium persists within macrophages in the mesenteric lymph nodes of chronically infected Nramp1+/+ mice and can be reactivated by IFN neutralization. J. Exper. Med. 2004, 199: 231-241.
- Martinoli C., Chiavelli A., Rescigno M. Entry route of Salmonella typhimurium directs the type of induced immune response. Immunity. 2007, 27 (6): 975-984.
- Nesterenko L.N., Balunets D.V., Tomova A.S. et al. Mycobacterium tuberculosis-susceptible I/St mice develop severe disease following infection with taxonomically distant bacteria, Salmonella enterica and Chlamydia pneumoniae. Clin. Exper. Immun. 2006, 146: 93-100.
- Sanchez F., Radaeva T.V., Nikonenko B.V. et al. Multigenic control of disease severity after virulent Mycobacterium tuberculosis infection in mice. Infect. Immun. 2003, 71: 126-131.
- van Spriel A.B., Sofi M., Gartlan K.H. et al. The tetraspanin protein CD37 regulates IgA responses and anti-fungal immunity. PLoS Pathog. 2009, 5 (3): e1000338.
Supplementary files
