Email this article UTOChNENIE TAKSONOMIChESKOGO POLOZhENIYa PROBIOTIChESKIKh ShTAMMOV RODA LACTOBACILLUS C POMOShch'Yu SEKVENIROVANIYa GENOV 16S rDNA i rpoA
- Authors: Voronina OL1, Kunda MS1, Bondarenko VM1, Shabanova NA1, Lunin VG1
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Affiliations:
- Issue: Vol 89, No 3 (2012)
- Pages: 18-24
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13666
- ID: 13666
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Abstract
Aim. Revision of the species identification of collection lactobacilli strains based on 16S rDNA
and rpoA gene sequencing. Materials and methods. 52 lactobacilli cultures that present mostly
Gamaleya Research Institute of Epidemiology and Microbiology (GIMC) collection were studied.
16S rDNA gene fragments were amplified by using Lb16a, Lb16b, 16S-midford, 16S-midrev
primers. 2 different reverse primers were used for the analysis of rpoA gene depending on lactobacilli
species. DNA fragments sequencing was performed with 3130 Genetic Analyzer (Applied
Biosystems/Hitachi) with primers used for amplification. Results. The effectiveness of sequencing
of 2 targets for differentiation of species within lactobacilli phylogenetic groups was shown. Species
diversity was demonstrated for GIMC lactobacilli strain collection that includes members of 9
species. All the strains marked previously as L. acidophilus were determined to belong to L. helveticus.
Strains belonging to recently discovered L. farraginis species that has promising application
in agriculture were detected. Conclusion. Genetic passports of original strains of 9 species of lactobacilli
that are promising for further research.
and rpoA gene sequencing. Materials and methods. 52 lactobacilli cultures that present mostly
Gamaleya Research Institute of Epidemiology and Microbiology (GIMC) collection were studied.
16S rDNA gene fragments were amplified by using Lb16a, Lb16b, 16S-midford, 16S-midrev
primers. 2 different reverse primers were used for the analysis of rpoA gene depending on lactobacilli
species. DNA fragments sequencing was performed with 3130 Genetic Analyzer (Applied
Biosystems/Hitachi) with primers used for amplification. Results. The effectiveness of sequencing
of 2 targets for differentiation of species within lactobacilli phylogenetic groups was shown. Species
diversity was demonstrated for GIMC lactobacilli strain collection that includes members of 9
species. All the strains marked previously as L. acidophilus were determined to belong to L. helveticus.
Strains belonging to recently discovered L. farraginis species that has promising application
in agriculture were detected. Conclusion. Genetic passports of original strains of 9 species of lactobacilli
that are promising for further research.
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