EFFECT OF INFLUENZA VACCINES ON SUBPOPULATIONS OF BLOOD DENDRITIC CELLS

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Aim. Study the effect of Vaxigrip split, Influvac subunit and Grippol plus immune-adjuvanted vaccines on the content of myeloid (mDC) and plasmacytoid (pDC) dendritic cells (DC) in blood of vaccinated healthy women. Materials and methods. Blood of 30 healthy women aged 18-50 years was studied at days 7 and 30 after the vaccination. pDC (CD14+CD16-/CD85k(ILT3)-PE/ CD123-PC5) and mDC (CD14+CD16-/CD85k(ILT3)-PE/CD33-PC5) immune phenotyping was carried out using mAbs (Beckman Coulter, France) and flow cytometer Cytomix FC-500 (Beckman Coulter, USA). Results. Use of unadjuvanted vaccines Vaxigrip and Influvac resulted in an increase of the numbers of mDC and pDC (p<0.05) in blood of the vaccinated only at day 7 of the observation. Grippol resulted in a more significant (2.2 - 3.6 times, p<0.05) increase of DC subpopulations (compared with unadjuvanted vaccines) at both day 7 and a month after the vaccination. Conclusion. Influenza vaccination activated innate effectors - the first component on the way of infection penetration - dendritic cells of both myeloid and lymphoid origin. Wherein, a more pronounced and prolonged effect of such activation is observed when immune-adjuvanted vaccine is used compared with subunit and split vaccines.

About the authors

E. A. Chromova

Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Russian Federation

E. A. Akhmatova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

S. A. Skhodova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

I. A. Semochkin

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

V. G. Khomenkov

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

N. K. Akhmatova

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

M. P. Kostinov

Mechnikov Research Institute of Vaccines and Sera

Russian Federation

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Copyright (c) 2016 Chromova E.A., Akhmatova E.A., Skhodova S.A., Semochkin I.A., Khomenkov V.G., Akhmatova N.K., Kostinov M.P.

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