MLVA-TYPING OF CLINICAL STAMPS OF GENETICALLY CHANGED VIBRIO CHOLERAE BIOTYPE EL TOR INSULATED IN RUSSIA AND UKRAINE IN THE PERIOD OF SEVENTH PANDEMIC CHOLERA

Abstract

Aim. Conduct in a comparative aspect MLVA-typing of genetically altered cholera vibrio biovar El Tor, isolated from patients during the epidemic (1994) and outbreaks (1993, 1998) in Dagestan with isolates in Mariupol (Ukraine) in 1994-2011 in Moscow (2010, 2012), India (1964, 2006, 2007), Bangladesh 1991, 1994, 2001, 2004) and to establish Phylogenetic connections between strains of cholera vibrios isolated in different years in these territories, to ascertain the source of their drift. Materials and methods. MLVA-tyP-ing was carried out in PCR at 5 variable loci of 35 clinical strains of genetically modified Vibrio cholerae byotyPe El Tor. The obtained amPlicon was studied in the system of automatic caPillary electroPhoresis ExPerion («Bio Rad Laboratories», USA). For Phylogenetic analysis, along with MLVA-genotyPes, 35 strains of Vibrio cholerae from the Institute's collection used Published genotyPes of strains isolated in India, Bangladesh, Haiti. Results. The investigated strains of cholera vibrio are referred to 21 MLVA-tyPes, divided into 2 main clades and 1 seParate branch with clonal clusters and subclusters, each of which contains closely related strains of cholera vibrio genovariants having a different degree of Phylogenetic relationshiP - full or Partial identity of allelic Profiles of five variable loci. The sources of drift of genetically modified Vibrio cholerae byotyPe El Tor to Russia and Ukraine from disadvantaged cholera of India, Bangladesh, Azerbaijan and the countries of the Middle East have been established. Conclusion. The obtained data testify to the PolymorPhism of MLVA-tyPes of genetically altered strains of cholera vibrio of the biologist El Tor, evolved in different years and caused ePidemics or outbreaks of cholera in different territories during different time Periods of the course of the seventh cholera Pandemic, and also suggest the Polyclonal origin of the Vibrio cholerae biovar El Tor and the source of their drift to the territory of the Russian Federation and Ukraine.

About the authors

I. V. Savelieva

Stavropol Research Institute for Plague Control

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

A. N. Kulichenko

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

V. N. Saveliev

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

D. A. Kovalev

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

O. V. Vasilieva

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. M. Zhirov

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

E. I. Eremenko

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

E. I. Podoprigora

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

B. V. Babenyshev

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

I. V. Kuznetsova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

L. V. Guseva

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2018 Savelieva I.V., Kulichenko A.N., Saveliev V.N., Kovalev D.A., Vasilieva O.V., Zhirov A.M., Eremenko E.I., Podoprigora E.I., Babenyshev B.V., Kuznetsova I.V., Guseva L.V.

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