METABOLIC ACTIVITY OF HIGH-ANTAGONISTIC STRAINS OF LACTOBACILLI ISOLATED FROM HEALTHY PEOPLE

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Aim. To evaluate the metabolic activity of highly antagonistic strains of lactobacilli isolated from the oral cavity and intestine of healthy people. Materials and methods. 9 highly antagonistically active strains of lactobacilli isolated from plaque and intestine of healthy people of different age groups from 8 to 35 years were included in the study. Enzymes of pathogenicity, acidproduc-tion, gasotransmitters (CH4, CO2, C2H6, CO и NH3) were studied in lactobacilli, as well as the degree of sensitivity to hydrochloric acid and bile. Results. All antagonistic strains of lactobacilli have been identified by 16S rRNA sequencing and assigned to 4 species: Lactobacillus fermentum, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei. All lactobacilli were apatogenic, producing a wide range of metabolites of varying severity: lactic acid and gasotransmitters, CH4, CO2, C2H6, CO и NH3. It was found that intestinal strains of lactobacilli were resistant to hydrochloric acid and bile than lactobacilli isolated from oral cavity. Conclusion. Highly antagonistic strains of lactobacilli have a wide spectrum of probiotic factors, they synthesize acids and gasotransmitters, persiste in the presence of hydrochloric acid and bile, and therefore they should be considered in the creation of new probiotics and functional products.

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V. M. Chervinets

Tver State Medical University

Author for correspondence.
Email: noemail@neicon.ru
Россия

Yu. V. Chervinets

Tver State Medical University

Email: noemail@neicon.ru
Россия

E. A. Belyaeva

Tver State Medical University

Email: noemail@neicon.ru
Россия

O. A. Petrova

Tver State Medical University

Email: noemail@neicon.ru
Россия

E. B. Ganina

Tver State Medical University

Email: noemail@neicon.ru
Россия

References

  1. Агапова О.В., Бондаренко В.М., Поликарпов Н.А. Ферменты патогенности клинических штаммов Klebsiella pneumoniae. Журн. микробиол. 1999, 2: 5-8.
  2. Гаврилова О.А., Червинец Ю.В. Содержимое зубодесневого желобка у дошкольников: физические и микробиологические характеристики. Стоматология детского возраста и профилактика. 2009, 2: 66-68.
  3. ГОСТ 33045-2014. Метод капиллярного электрофореза с использованием системы капиллярного электрофореза «Капель». ПНД Ф 14.1:2:4.167-2000. М., 2000.
  4. Червинец Ю.В. и др. Генетическая идентификация антагонистически активных штаммов лактобацилл, выделенных из полости рта здоровых людей. Клиническая лабораторная диагностика. 2010, 11: 43-46.
  5. Червинец Ю.В. и др. Индигенные лактобациллы полости рта человека - кандидаты в пробиотические штаммы. Курский научно-практический вестник «Человек и здоровье». 2012, 1: 131-137.
  6. Червинец В.М. и др. Пробиотический и адаптационный потенциал лактобацилл, перспективных для конструирования эффективных пробиотических препаратов. Экспериментальная и клиническая гастроэнтерология. 2016, 126 (2): 108.
  7. Шендеров Б.А. Роль эндогенных и микробных газовых молекул в физиологии патофизиологии сердечно-сосудистой системы. Вестник восстановительной медицины. 2015, 5: 58-65.
  8. Althaus M., Clauss W.G. Gasotransmitters: novel regulators of ion channels and transporters. Front Physiol. 2013, 4: 27. doi: 10.3389/fphys.2013.00027.
  9. Casey P.G., Casey G.D., Gardiner G.E. et al. Isolation and characterization of anti-Salmonella lactic acid bacteria from the porcine gastrointestinal tract. Lett. Appl. Microbiol. 2004, 39: 431-438.
  10. Lebeer S., Vanderleyden J., De Keersmaeker S.C.J. Genes and molecules of lactobacilli supporting probioticaction. Microbiol Mol. Biol. Rev. 2008. 72: 728-764.
  11. Mikelsaar M., Sepp E., Stsepetova J. et al. Biodiversity of Intestinal Lactic Acid Bacteria in the Healthy Population. Advances in Microbiology, Infectious Diseases and Public Health. 2016, 4: 1-64. doi: 10.1007/5584_2016_3.
  12. MU 2.3.2.2789-10 Guidelines for the sanitary-epidemiological assessment of the safety and functional potential of probiotic microorganisms used for food production. Food Raw Materials and Food Products. 2010, Moscow (in Russia).
  13. Shenderov B.A. Probiotics and Functional Foods, 2011. In Food Engineering, Encyclopedia of Life Support Systems (EOLSS). Developed under the Auspices of the uNesCo, Eolss Publishers, Oxford, UK. (http:www. eolss.net).
  14. Sasithorn Sirilun, Chaiyavat Chaiyasut, Duangporn Kantachote, Plearnpis Luxananil Characterisation of non-human origin probiotic Lactobacillus plantarum with cholesterollowering property. African J. Microbiology Research. 2010, 4 (10): 994-1000.
  15. Tinajero-Trejo M., Jesse H.E., Poole R.K. Gasotransmitters, poisons, and antimicrobials: it’s a gas, gas, gas! F1000Prime Report. 2013, 5: 28. doi: 10.12703/P5-28.

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Copyright (c) 2018 Chervinets V.M., Chervinets Y.V., Belyaeva E.A., Petrova O.A., Ganina E.B.

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