METABOLIC ACTIVITY OF HIGH-ANTAGONISTIC STRAINS OF LACTOBACILLI ISOLATED FROM HEALTHY PEOPLE

Aim. To evaluate the metabolic activity of highly antagonistic strains of lactobacilli isolated from the oral cavity and intestine of healthy people. Materials and methods. 9 highly antagonistically active strains of lactobacilli isolated from plaque and intestine of healthy people of different age groups from 8 to 35 years were included in the study. Enzymes of pathogenicity, acidproduc-tion, gasotransmitters (CH₄, CO₂, C₂H₆, CO и NH₃) were studied in lactobacilli, as well as the degree of sensitivity to hydrochloric acid and bile. Results. All antagonistic strains of lactobacilli have been identified by 16S rRNA sequencing and assigned to 4 species: Lactobacillus fermentum, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei. All lactobacilli were apatogenic, producing a wide range of metabolites of varying severity: lactic acid and gasotransmitters, CH₄, CO₂, C₂H₆, CO и NH₃. It was found that intestinal strains of lactobacilli were resistant to hydrochloric acid and bile than lactobacilli isolated from oral cavity. Conclusion. Highly antagonistic strains of lactobacilli have a wide spectrum of probiotic factors, they synthesize acids and gasotransmitters, persiste in the presence of hydrochloric acid and bile, and therefore they should be considered in the creation of new probiotics and functional products.

lactobacilli, oral cavity, intestine, acid production, persistence, gasotransmitters