AN OCCURRENCE OF ICE-emm12 GENETIC ELEMENT CONTAINING tetM AND ermB RESISTANCE GENES AMONG RUSSIAN AND VIETNAMESE GROUP A STREPTOCOCCAL STRAINS

Cover Page


Cite item

Full Text

Abstract

Aim. Goal of the study is characterization and analysis of an occurrence of ICE-emm12 genetic element associated with streptococcal outbreaks, among Vietnamese and Russian GAS strains. Materials and methods. A total of 96 strains isolated in 2007 - 2014 in Moscow, Saint-Petersburg and different provinces of Vietnam were studied. Molecular genetic experiments were done as previously described. Whole genome sequencing was done using MiSeq technology. Results. Complete genome sequencing of Vietnamese strain V31 revealed the presence of 61028 bp fragment homologous to integrative and conjugative element ICE-emm12 containing resistance genes to MLS-antibiotics (ermB) and tetracycline (tetM). This element was discovered in 12 (26,1%, types emm12.0, emm12.22) out of 46 Vietnamese strains, and 2 (4,0%, types emm12.0, emm88.2) out of 50 Russian strains. In 13 out of 14 strains, ICE-emm12 was integrated in RNA-methyltransferase gene, which is possibly the «hot spot» for recombination. In all the strains ICE-emm12 was present in two forms: integrated linearized form and excised circular form with potential to be horizontally transferred. Conclusion. The presence of ICE-emm12 containing antibiotic resistance genes and associated with streptococcal outbreaks in South East Asia, among Russian GAS strains together with the fact of intensive tourism industry indicate the need of molecular epidemiological surveillance for circulation of epidemiologically actual streptococcal clones in Russia.

About the authors

A. G. Kireeva

Institute of Experimental Medicine; Saint-Petersburg State University

Author for correspondence.
Russian Federation

O. V. Kalinina

Almazov North-West Federal Medical Research Centre; Saint-Petersburg State Technological Institute (Technical University)

Russian Federation

A. M. Kiselev

Almazov North-West Federal Medical Research Centre

Russian Federation

N. I. Briko

Sechenov First Moscow State Medical University

Russian Federation

E. V. Glushkova

Sechenov First Moscow State Medical University

Russian Federation

A. V. Dmitriev

Institute of Experimental Medicine; Saint-Petersburg State University

Russian Federation

References

  1. Носик А.Г., Ильясов Ю.Ю., Линь Ф.К., Дмитриев А.В. Молекулярно-эпидемиологическая характеристика стрептококков, выделенных у детей младшего школьного возраста во Вьетнаме. Журн. инфектологии. 2015, 7 (3): 112-118.
  2. Полякова Е.М., Мнацаканян Е.С., Бурова Л.А., Дмитриев А.В. Генетическая гетерогенность штаммов Streptococcus pyogenes генотипа emm12. Медицинский академический журнал. 2010, 10 (1): 39-44.
  3. Beres S.B., Musser J.M. Contribution of exogenous genetic elements to the group A Streptococcus metagenome. PLoS One. 2007, 2 (8): e800.
  4. Carapetis J.R., Steer A.C., Mulholland E.K. et al. The global burden of group A streptococcal diseases. Lancet Infectious Diseases. 2005, 5 (11): 685-694.
  5. Elliot J.A., Farmer K.D., Facklam R.R. Sudden increase in isolation of group B streptococci, serotype V, is not due to emergence of a new pulsed-field gel electrophoresis type. J. Clin. Microbiol. 1998, 36: 2115-2116.
  6. Feng L.J., Lin H.R., Ma Y.L. et al. Macrolide-resistant Streptococcus pyogenes from Chinese pediatric patients in association with Tn916 transposons family over a 16-year period. Diagnostic Microbiology Infectious Disease. 2010, 67 (4): 369-375.
  7. Guy R., Williams C., Irvine N. et al. Increase in scarlet fever notifications in the United Kingdom, 2013/14. Eurosurveillance. 2014, 19 (12): 20749.
  8. Ip M., Lyon D.J., Leung T. et al. Macrolide resistance and distribution of erm and mef genes among beta-haemolytic streptococci in Hong Kong. European J. Clinical Microbiology Infectious Diseases. 2002, 21 (3): 238-240.
  9. Kataja J., Huovinen P., Seppala H. Macrolide resistance study G. Erythromycin resistance genes in group A streptococci of different geographical origins. J. Antimicrobial Chemotherapy. 2000, 46 (5): 789-792.
  10. Luk E.Y., Lo J.Y., Li A.Z. et al. Scarlet fever epidemic, Hong Kong, 2011. Emerging Infectious Diseases. 2012, 18 (10): 1658-1661.
  11. Olivieri R., Morandi M., Zanchi A. et al. Evolution of macrolide resistance in Streptococcus pyogenes over 14 years in an area of central Italy. J. Medical Microbiology. 2015, 64: 11861195.
  12. ProMED-Mail (2009, June 20). Streptococcus, group A, scarlet fever - Vietnam. Retrieved December 06, 2015.
  13. Tse H., Bao J.Y.J., Davies M.R. et al. Molecular сharacterization of the 2011 Hong Kong Scarlet Fever Outbreak. J. Infectious Diseases. 2012, 206 (3): 341-351.
  14. Varaldo P.E., Montanari M.P., Giovanetti E. Genetic elements responsible for erythromycin resistance in streptococci, Antimicrobial Agents Chemotherapy. 2009, 53: 343-353.
  15. Yan J.J., Wu H.M., Huang A.H. et al. Prevalence of polyclonal mefA-containing isolates among erythromycin-resistant group A streptococci in southern Taiwan. J. Clinical Microbiology. 2000, 38 (7): 2475-2479.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Kireeva A.G., Kalinina O.V., Kiselev A.M., Briko N.I., Glushkova E.V., Dmitriev A.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.