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Aim. Isolation and study ofbiological activity of antimicrobial peptides from chickens thrombocytes. Materials and methods. Peptides from chickens thrombocytes, obtained by reverse-phase high-performance liquid chromatography method with stepped and linear gradients of concentration increase of the organic solvent were used in the study. Their antimicrobial activity was determined by microtitration method in broth; mechanism of biological effect - by using fluorescent spectroscopy method with DNA-tropic dyes. Results. Individual fractions of peptides were isolated from chickens thrombocytes, that possess antimicrobial activity against Staphylococcus aureus P209 and Escherichia coli K12. A disruption of integrity of barrier structures of microorganisms under the effect of thrombocyte antimicrobial peptides and predominance of cells with damaged membrane in the population of E. coli was established. Conclusion. The data obtained on antimicrobial activity and mechanism of bactericidal effect of the peptide fractions from chickens thrombocytes isolated for the first time expand the understanding of functional properties of chickens thrombocytes and open a perspective for their further study with the aim of use as antimicrobial means.

About the authors

M. V. Sycheva

Research Institute of Cellular and Intracellular Symbiosis; Orenburg State Agrarian University

Author for correspondence.
Russian Federation

A. S. Vasilchenko

Research Institute of Cellular and Intracellular Symbiosis; Orenburg State University

Russian Federation

E. A. Rogozhin

Shemyakin and Ovchinnikov Research Institute of Bioorganic Chemistry

Russian Federation

T. M. Pashkova

Research Institute of Cellular and Intracellular Symbiosis

Russian Federation

L. P. Popova

Research Institute of Cellular and Intracellular Symbiosis

Russian Federation

O. L. Kartashova

Research Institute of Cellular and Intracellular Symbiosis

Russian Federation


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Copyright (c) 2016 Sycheva M.V., Vasilchenko A.S., Rogozhin E.A., Pashkova T.M., Popova L.P., Kartashova O.L.

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