Email this article BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES FROM CHICKENS THROMBOCYTES
- Authors: Sycheva M.V.1,2, Vasilchenko A.S.1,3, Rogozhin E.A.4, Pashkova T.M.1, Popova L.P.1, Kartashova O.L.1
-
Affiliations:
- Research Institute of Cellular and Intracellular Symbiosis
- Orenburg State Agrarian University
- Orenburg State University
- Shemyakin and Ovchinnikov Research Institute of Bioorganic Chemistry
- Issue: Vol 93, No 2 (2016)
- Pages: 24-29
- Section: ORIGINAL RESEARCHES
- URL: https://microbiol.crie.ru/jour/article/view/26
- DOI: https://doi.org/10.36233/0372-9311-2016-2-24-29
- ID: 26
Cite item
Full Text
Abstract
Aim. Isolation and study ofbiological activity of antimicrobial peptides from chickens thrombocytes. Materials and methods. Peptides from chickens thrombocytes, obtained by reverse-phase high-performance liquid chromatography method with stepped and linear gradients of concentration increase of the organic solvent were used in the study. Their antimicrobial activity was determined by microtitration method in broth; mechanism of biological effect - by using fluorescent spectroscopy method with DNA-tropic dyes. Results. Individual fractions of peptides were isolated from chickens thrombocytes, that possess antimicrobial activity against Staphylococcus aureus P209 and Escherichia coli K12. A disruption of integrity of barrier structures of microorganisms under the effect of thrombocyte antimicrobial peptides and predominance of cells with damaged membrane in the population of E. coli was established. Conclusion. The data obtained on antimicrobial activity and mechanism of bactericidal effect of the peptide fractions from chickens thrombocytes isolated for the first time expand the understanding of functional properties of chickens thrombocytes and open a perspective for their further study with the aim of use as antimicrobial means.
About the authors
M. V. Sycheva
Research Institute of Cellular and Intracellular Symbiosis; Orenburg State Agrarian University
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
A. S. Vasilchenko
Research Institute of Cellular and Intracellular Symbiosis; Orenburg State University
Email: noemail@neicon.ru
Russian Federation
E. A. Rogozhin
Shemyakin and Ovchinnikov Research Institute of Bioorganic Chemistry
Email: noemail@neicon.ru
Russian Federation
T. M. Pashkova
Research Institute of Cellular and Intracellular Symbiosis
Email: noemail@neicon.ru
Russian Federation
L. P. Popova
Research Institute of Cellular and Intracellular Symbiosis
Email: noemail@neicon.ru
Russian Federation
O. L. Kartashova
Research Institute of Cellular and Intracellular Symbiosis
Email: noemail@neicon.ru
Russian Federation
References
- Резистентность к противомикробным препаратам: повторение «трагедии общего достояния». Бюллетень ВОЗ. 2010, 88 (11): 805-806.
- Aktan I., Dunkel B., Cunningham F.M. Equine platelets inhibit E. coli growth and can be activated by bacterial lipopolysaccharide and lipoteichoic acid although superoxide anion production does not occur and platelet activation is not associated with enhanced production by neutrophils. Veterinary Immunol. Immunopathol. 2013, 152 (3-4): 209-217.
- Aziz A.-M. The role of healthcare strategies in controlling antibiotic resistance. British J. Nursing. 2013, 22 (18): 1066-1074.
- Brodgen K.F. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat. Rev. Microbiol. 2005, 3 (3): 238-250.
- Dankert J., Krijgsveld J., Van der Werff J. et al. Platelet microbicidal activity is an important defense factor against viridans Streptococcal endocarditis. J. Infect. Dis. 2001, 184: 597-605.
- Fleming A. On the antibacterial action of cultures of a penicillium, with special reference to their use in the isolation of B. Influenzae. Br. J. Exp. Pathol. 1929, 10: 226-236.
- Forde E., Devocelle M. Pro-moieties of antimicrobial peptide prodrugs. Molecules. 2015, 20 (1): 1210-1227.
- Ivanov I.B., Gritsenko V.A. Comparative activities of cattle and swine platelet microbicidal proteins. Probiotics Antimicrob. Proteins. 2009, 1 (2): 148-151.
- Jenssen H., Hamill P., Hancock R.E.W. Peptide antimicrobial agents. Clin. Microbiol. Rev. 2006, 19 (3): 491-511.
- Mohan K.V.K., Rao S.S., Gao Y. et al. Enhanced antimicrobial activity of peptide-cocktails against common bacterial contaminants of ex vivo stored platelets. Clin. Microbiol. Infect. 2014, 20 (1): 39-46.
- Tang Y.-Q., Yeaman M.R., Selsted M.E. Antimicrobial peptides from human platelets. Infect. Immunity. 2002, 70 (12): 6524-6533.
- Vasilchenko A., Dymova V., Kartashova O. et al. Morphofunctional reaction of bacteria treated with antimicrobial peptides derived from farm animal platelets. Probiotics Antimicrob. Proteins. 2015, 7 (1): 60-65.
- Wang G. Human antimicrobial peptides and proteins. Pharmaceuticals. 2014, 7 (5): 545594.
- Wiegand I., Hilpert K., Hancock R.E.W. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols. 2008, 3 (2): 163-175.
- Yamasaki K., Gallo R.L. Antimicrobial peptides in human skin disease. Eur. J. Dermatol. 2008, 18 (1): 11-21.
- Yeaman M.R. Platelets: At the nexus of antimicrobial defence. Nature Rev. Microbiol. 2014, 12 (6): 426-437.
- Yount N.Y, Gank K.D., Xiong YQ. et al. Platelet microbicidal protein 1: Structural themes of a multifunctional antimicrobial peptide. Antimicrob. Agents Chemother. 2004, 48 (11): 4395-4404.
- Yurong Y., Yibao J., Ruiping S. et al. Effects of chicken intestinal antimicrobial peptides on humoral immunity of chickens and antibody titres after vaccination with infectious bursal disease virus vaccine in chicken. Archives Animal Nutrition. 2006, 60 (5): 427-435.
- Zhu X., Dong N., Wang Z. et al. Design of imperfectly amphipathic a-helical antimicrobial peptides with enhanced cell selectivity. Acta Biomaterialia. 2014, 10 (1): 244-257.
Supplementary files
