APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

D. V. Ulshina; Ульшина Д. В.; D. A. Kovalev; Ковалев Д. А.; D. G. Ponomarenko; Пономаренко Д. Г.; D. V. Rusanova; Русанова Д. В.; N. M. Shvetsova; Швецова Н. М.; T. V. Taran; Таран Т. В.; I. V. Kuznetsova; Кузнецова И. В.; A. M. Zhirov; Жиров А. М.; A. A. Khachaturova; Хачатурова А. А.; I. Yu. Borzdova; Борздова И. Ю.; A. N. Kulichenko; Куличенко А. Н.

doi:10.36233/0372-9311-2017-4-9-17

APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

Authors: Ulshina D.V.¹, Kovalev D.A.¹, Ponomarenko D.G.¹, Rusanova D.V.¹, Shvetsova N.M.¹, Taran T.V.¹, Kuznetsova I.V.¹, Zhirov A.M.¹, Khachaturova A.A.¹, Borzdova I.Y.¹, Kulichenko A.N.¹
Affiliations:
1. Stavropol Research Institute for Plague Control
Issue: Vol 94, No 4 (2017)
Pages: 9-17
Section: ORIGINAL RESEARCHES
URL: https://microbiol.crie.ru/jour/article/view/172
DOI: https://doi.org/10.36233/0372-9311-2017-4-9-17
ID: 172

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Abstract

Aim.Study the possibility to apply time-of-flight mass-spectrometry for detection of causative agent of brucellosis in blood. Materials and methods. Brucella strains: 5 Brucella melitensis and 21 Brucella abortus. Protein profiling in linear mode on MALD1-TOF mass-spectrometer Microflex «Bruker Daltonics». Results. Technique for disinfection and preparation of blood samples was modified and optimized for MALDI-TOF MS analysis. 120 representative protein profiles of sera extract were obtained that contain brucellosis causative agent. A resulting peak-list (super-spectrum) of the studied protein fraction of blood extract of a conditionally healthy human within the studied group was formed and analyzed. Conclusion. A scheme of brucella detection in blood samples by MALDI-TOF MS is proposed, based on detection of a complex of 15 genus-specific fragments. Signals on mass-spectra of extracts of leukocyte fraction of blood, artificially contaminated with brucellosis causative agents are characterized.

Keywords

brucellosis causative agent, blood, mass-spectrometry, protein profiling, detection

References

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APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

Full Text

Abstract

Keywords

About the authors

References

Supplementary files

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