APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

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Abstract

Aim.Study the possibility to apply time-of-flight mass-spectrometry for detection of causative agent of brucellosis in blood. Materials and methods. Brucella strains: 5 Brucella melitensis and 21 Brucella abortus. Protein profiling in linear mode on MALD1-TOF mass-spectrometer Microflex «Bruker Daltonics». Results. Technique for disinfection and preparation of blood samples was modified and optimized for MALDI-TOF MS analysis. 120 representative protein profiles of sera extract were obtained that contain brucellosis causative agent. A resulting peak-list (super-spectrum) of the studied protein fraction of blood extract of a conditionally healthy human within the studied group was formed and analyzed. Conclusion. A scheme of brucella detection in blood samples by MALDI-TOF MS is proposed, based on detection of a complex of 15 genus-specific fragments. Signals on mass-spectra of extracts of leukocyte fraction of blood, artificially contaminated with brucellosis causative agents are characterized.

About the authors

D. V. Ulshina

Stavropol Research Institute for Plague Control

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

D. A. Kovalev

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

D. G. Ponomarenko

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

D. V. Rusanova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

N. M. Shvetsova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

T. V. Taran

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

I. V. Kuznetsova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. M. Zhirov

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. A. Khachaturova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

I. Yu. Borzdova

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

A. N. Kulichenko

Stavropol Research Institute for Plague Control

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2017 Ulshina D.V., Kovalev D.A., Ponomarenko D.G., Rusanova D.V., Shvetsova N.M., Taran T.V., Kuznetsova I.V., Zhirov A.M., Khachaturova A.A., Borzdova I.Y., Kulichenko A.N.

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