Email this article ANTIVIRAL ACTIVITY OF POLYPRENYLPHOSPHATES IN EXPERIMENTAL INFECTION CAUSED BY HEPATITIS C VIRUS IN VITRO
- Authors: Narovlyanskiy AN1, Deryabin PG1, Sedov AM1, Sanin AV1, Pronin AV1
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Affiliations:
- Issue: Vol 89, No 5 (2012)
- Pages: 80-84
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14219
- ID: 14219
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Abstract
Aim. Study antiviral effect of sodium polyprenylphosphate (PPP) in experimental infection model caused
by hepatitis C virus (HCV) in cell culture. Materials and methods. Cytopathogenic variant of HCV isolated
from blood serum of a chronically infected patient was used. HCV infectious dose was 10.0 TCD50. Highly
sensitive to cytopathogenic effect of HCV continuous swine embryo kidney cells (SPEV) as 1 day monolayer
grown in 24 well plastic plates on 199 medium with 10% calf serum with addition of L-glutamine and
antibiotics (100 U/ml) were used. PPP was used in concentrations that do not have cytotoxic effect (from 60
to 7.5 g in 50 l); introduced into SPEV cell cultures immediately after infection, 24 hours before or 24
hours after the infection of cells with HCV. Infectious activity of HCV was evaluated by using Reed-Muench
formula based on results of medium samples titration obtained 3 days after the infection of cells. Results. PPP
was shown to have antiviral properties when added into the cell cultures immediately after the infection with
HCV. Under the effect of PPP HCV titers were established to decrease by 3.0 lg (PPV dose of 60 g) and by
1.9 lg (PPV dose of 30 g). Positive effect was also obtained for prophylactic use of PPP. When PPP at a dose
of 60 g was introduced 24 hours before the infection of SPEV with HCV, the titer of the virus decreased by
3.5 lg. Prophylactic administration of low doses of the preparation (7.5 and 15.0 g) also showed evident
antiviral effect (decrease of infectious activity of HCV by 3.2 - 2.3 lg, respectively). Conclusion. PPP at the
doses tested has an ability to reduce concentration of HCV in SPEV cell cultures when added immediately
after infection or 24 hours before.
by hepatitis C virus (HCV) in cell culture. Materials and methods. Cytopathogenic variant of HCV isolated
from blood serum of a chronically infected patient was used. HCV infectious dose was 10.0 TCD50. Highly
sensitive to cytopathogenic effect of HCV continuous swine embryo kidney cells (SPEV) as 1 day monolayer
grown in 24 well plastic plates on 199 medium with 10% calf serum with addition of L-glutamine and
antibiotics (100 U/ml) were used. PPP was used in concentrations that do not have cytotoxic effect (from 60
to 7.5 g in 50 l); introduced into SPEV cell cultures immediately after infection, 24 hours before or 24
hours after the infection of cells with HCV. Infectious activity of HCV was evaluated by using Reed-Muench
formula based on results of medium samples titration obtained 3 days after the infection of cells. Results. PPP
was shown to have antiviral properties when added into the cell cultures immediately after the infection with
HCV. Under the effect of PPP HCV titers were established to decrease by 3.0 lg (PPV dose of 60 g) and by
1.9 lg (PPV dose of 30 g). Positive effect was also obtained for prophylactic use of PPP. When PPP at a dose
of 60 g was introduced 24 hours before the infection of SPEV with HCV, the titer of the virus decreased by
3.5 lg. Prophylactic administration of low doses of the preparation (7.5 and 15.0 g) also showed evident
antiviral effect (decrease of infectious activity of HCV by 3.2 - 2.3 lg, respectively). Conclusion. PPP at the
doses tested has an ability to reduce concentration of HCV in SPEV cell cultures when added immediately
after infection or 24 hours before.
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