Email this article ISOLATION OF MYCOBACTERIA ON DIFFERENT GROWTH MEDIA AND THEIR IDENTIFICATION
- Authors: Makarova M.V1, Levchenko T.N1, Freiman G.E1
-
Affiliations:
- Moscow Scientific and Practical Center for Tuberculosis Control, Russia
- Issue: Vol 86, No 3 (2009)
- Pages: 7-10
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14180
- ID: 14180
Cite item
Full Text
Abstract
Aim. To compare results of isolation of mycobacteria on different growth media. Materials and methods. From August 2005 to September 2008 Central Bacteriological Laboratory of MSPCTC performed 111,029 inoculations of clinical samples, isolated 14,513 (13.5%) cultures of mycobacteria, of which 14,095 (97.1%) belonged to M.tuberculosis complex and 418 (2.9%) — to non-tuberculous mycobacteria (NTM). Results. Two thirds of isolated NTM belonged to slowly growing NTM (Ranyon classification), of which bacteria from M.avium complex as well as M.kansasii and M.xenopi predominated. M.fortuitum was the most frequently isolated between rapidly growing NTM. Conclusion. For isolation and identification of NTM the optimal was inoculation on at least 2 media: solid agar (Middlebrook 7H11) or egg medium and liquid medium (Middlebrook 7H9, in automated system BACTEC MGIT 960), each of which has its own advantages.
Keywords
About the authors
M. V Makarova
Moscow Scientific and Practical Center for Tuberculosis Control, Russia
T. N Levchenko
Moscow Scientific and Practical Center for Tuberculosis Control, Russia
G. E Freiman
Moscow Scientific and Practical Center for Tuberculosis Control, Russia
References
- Вейсфейлер Ю.К. Биология и изменчивость микобактерий туберкулеза и атипичные микобактерии. Экспериментальные и теоретические исследования. Будапешт, Академия наук Венгрии, 1975.
- Дыхно М.М. Сравнительное изучение и методы дифференциации туберкулезных микобактерий, атипичных штаммов и кислотоустойчивых сапрофитов. Дис. д-ра. мед. наук. М., 1963.
- Зыков М.П., Ильина Т.Б. Потенциально патогенные микобактерии и лабораторная диагностика микобактериозов. М., Медицина, 1978.
- Макаревич Н.М. Атипичные микобактерии: методы идентификации, источники выделения, значение в клинике. Дис. д-ра мед. наук. М., 1973.
- Оттен Т.Ф., Васильев А.В. Микобактериоз. СПб, Мед. пресса, 2005.
- Adle-Biassette H., Huerre M., Breton G. et al. Non-tuberculous mycobacterial diseases. Ann. Pathol. 2003, 23: 216—235.
- Heifets L. Mycobacterial infections caused by nontuberculous mycobacteria. Sem. Respir. Crit. Care Med. 2004, 25 (3): 283—295.
- Heifets L., Jenkins P. Speciation of micobacteria in clinical laboratories. In: P.R.Gangadharam, P.A.Jenkins (ed.). Mycobacteria. New York, Chapman a Hall (Inf.Thompson Publishing), 1998, p. 308—350.
- Katoch V. Infections due to non-tuberculous mycobacteria (NTM). Indian J. Med. Res. 2004, 120: 290—304.
- Katoch V. Kumar M. Atipical mycobacterial infections. In: S.K.Sharma (ed.). Tuberculosis 1st ed. New Delhi Jaypece Bradhers Medical Publishers Ltd. 2001, p. 439—451.
- Koh W., Kwon O., Lee K. Nontuberculous mycobacterial pulmonary diseases in immunocompetent patients. Korean J. Radiol. 2002, 3: 145—157.
- Lu D.,Heeren B., Dunne M. Comparison of the automated mycobacteria growth indicator tube system (bactec 960/mgit) with Lowenstein-Jensen medium for recovery of mycobacteria from clinical specimens. Am.J.Clin.Pathol. 2002, 118: 542—545.
- Wallace R., Glassroth J., Griffith D. et al. Diagnosis and treatment of disease caused by nontuberculous mycobacteria (American Thoracic Society Statement). Am. J. Respir. Crit. Care Med. 1997, 156: 1—25.
- Wilson M., Stone B., Hildred M. et al. Comparison of recovery rates for mycobacteria from BACTEC 12B vials, Middlebrook 7H11selective 7H11 biplates, and Lowenstein Jensen slants in public health mycobacteriology laboratory. J. Clin. Microbiol. 1995, 33: 2516—2518.
Supplementary files
