Email this article MORPHOLOGIC CHANGES IN LISTERIA CELLS UNDER THE EFFECT OF METABOLITES OF HUMAN INTESTINAL MICROFLORA ENTEROCOCCI
- Authors: Valyshev A.V1, Vasilchenko A.S1
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Affiliations:
- Research Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
- Issue: Vol 91, No 6 (2014)
- Pages: 78-81
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/14036
- ID: 14036
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Abstract
Aim. Characterization of morphologic changes in listeria cells under the effect of metabolites ofhuman intestine microflora enterococci. Materials and methods. Culture of Listeria monocytogenes 88-BK cells was cultivated in the presence of enterococci waste products (experiment) or without them (control). The samples obtained were studied by atomic-force microscopy (AFM) in contact mode using scanning probe microscope SMM-2000. Results. The character of morpho-functional reaction of indicator culture bacterial cells allows to assume the presence of cationic anti-microbial peptides with membrane-lytic mechanism of action in 30% of the studied enterococci strains. Conclusion. The use of atomic-force microscopy allows to detail the mechanisms of antimicrobial activity of bacteriocins of intestine microflora microorganisms against target bacteria under close to native conditions.
About the authors
A. V Valyshev
Research Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
A. S Vasilchenko
Research Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia
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