STUDY OF PROTECTIVE ACTIVITY OF «STAPHYLOVAC-2» VACCINE


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Abstract

Aim. Study the protective properties of«Staphylovac-2» vaccine. Materials and methods. Samples of the vaccine manufactured by SPA «Microgen» based on the developed technology were studied in balb/c mice during 3- and 6-fold immunization schemes. Protective activity of the preparation was determined in experiments with active and passive protection during intraperitoneal infection, seeding of the causative agent from spleen and kidneys during intravenous infection of animals. Results. In experiments with active protection ofmice for both 3- and 6-fold immunization schemes, a significant protective activity of the studied series was determined compared with the control group of mice. Sera obtained after animal immunization (rabbits, mice) by staphylococcus vaccine had protective properties. A reduction of spleen and kidneys seeding by Staphylococcus aureus in immunized mice compared with the control group was detected in the model of generalized staphylococci infection. Conclusion. The preclinical studies carried out with the «Staphylovac-2» vaccine, developed based on the complex of protective staphylococci antigens, have confirmed the high protective activity of the preparation.

About the authors

I. M Gruber

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. B Egorova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

N. A Mikhailova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

L. S Cherkasova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

O. E Tarasova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. A Astashkina

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

O. M Ignatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

E. A Kurbatova

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

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Copyright (c) 2014 Gruber I.M., Egorova N.B., Mikhailova N.A., Cherkasova L.S., Tarasova O.E., Astashkina E.A., Ignatova O.M., Kurbatova E.A.

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