PREVALENCE OF CYTOTOXICITY EFFECTORS IN NOSOCOMIAL PSEUDOMONAS AERUGINOSA STRAINS


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Abstract

Aim. Analysis of occurrence of the third type secretory system (TTSS) effectors in clinical P. aeruginosa strains. Materials and methods. Intra-hospital (n=164) and extra-hospital (n=30) strains of P. aeruginosa were studied. Detection of exoS and exoU genes was carried out by PCR in DNA Engine Dyad Thermal Cycler («Bio-Rad», USA). Metallo-beta-lactamase (MBL) producers were detected by the presence of blaVIM-2 gene. Results. Screening of intra- and extra-hospital strains for the presence of genes coding ExoS and ExoU showed, that exoS is detected in genome of clinical isolates in 59.8% and exoU - 31.1% of cases. At the same time, strains with exoS-/exoU+ genotype predominated in ICU (ф=0.466; p=0.0000). A significant association between the presence of the respective effectors and material of strain isolation was not detected. exoU gene was more frequently detected in genome of MBL producers (ф=0.784; p=0.0004). Conclusion. A significant association between exoU and blaVIM-2 could be explained by clonal prevalence of P. aeruginosa ST235 VIM-2, circulation of those is noted on all the territory of Russia. As a rule, ExoU is produced by highly virulent poly-antibiotic resistant hospital isolates that determine unfavorable outcomes of pseudomonas infection.

About the authors

M. V Kuznetsova

Research Institute of Ecology and Genetics of Microorganisms, State Medical Academy, Perm, Russia

A. V Maksimova

Research Institute of Ecology and Genetics of Microorganisms, Perm, Russia

T. I Karpunina

State Medical Academy, Perm, Russia

V. A Demakov

Research Institute of Ecology and Genetics of Microorganisms, Perm, Russia

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Copyright (c) 2014 Kuznetsova M.V., Maksimova A.V., Karpunina T.I., Demakov V.A.

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