MULTILOCUS SEQUENCING OF CORYNEBACTERIUM DIPHTHERIAE STRAINS ISOLATED IN RUSSIA IN 2002 - 2012


Cite item

Full Text

Abstract

Aim. Characterization of contemporary С. diphtheriae strains isolated in Russia by using multilocus DNA sequencing (MLST). Materials and methods. 28 toxigenic C. diphtheriae strains isolated in Russia in 2002-2012 and sent to diphtheria and pertussis reference center of Gabrichevsky Research Institute of Epidemiology and Microbiology were studied. С. diphtheriae strain genotyp-ing was performed by using MLST based on atpA, dnaE, dnaK, fusA, leuA, odhA and rpoB gene fragments. Identification of alleles and ST was carried out according to EMBL/GenBank and PubMLST, eBurst approach was used for cluster analysis. Results. By using MLST contemporary toxigenic C. diphtheriae strains isolated in Russia in 2002-2012 were characterized. 8 genotypes (ST41, ST5, ST8, ST28, ST25, ST44, ST-new1 and ST-new2) were identified, 3 among them were dominating - ST8, ST28 and ST-new1. Most of the toxigenic strains belong to biovar gravis and ST8. Among biovar mitis strains a higher heterogeneity by ST membership was noted, but with prevalence of ST28 strains. Conclusion. Use of MLST allowed to characterize contemporary circulating population of toxigenic C. diphtheriae strains isolated in Russia and showed perspective of application of this method for characterization of diphtheria causative agent population and detection of epidemically significant strains, as well as juxtaposing of them with genetic structure of foreign strains.

About the authors

O. Yu Borisova

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia

I. K Mazurova

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia

I. A Chagina

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia

A. S Pimenova

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia

E. E Donskikh

Pirogov Russian Research Medical University, Moscow, Russia

V. A Aleshkin

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia

References

  1. Комбарова С.Ю., Мазурова И.К., Мельников В.Г. и др. Генетическая структура штаммов Corynebacterium diphtheriae, выделенных в России при разной интенсивности эпидемического процесса дифтерии. Журн. микробиол. 2001, 3: 3-8.
  2. Комбарова С.Ю. Микробиологический молекулярно-генетический мониторинг возбудителя дифтерийной инфекции. Автореф. дис. д-ра биол. наук, 2007.
  3. Комбарова С.Ю., Борисова О.Ю., Мельников В.Г. и др. Наблюдение за циркуляцией штаммов C.diphtheriae, различающихся по признаку токсигенности. Медицинский альманах. 2009, 2 (7): 108-111.
  4. Мазурова И.К. Комплексная система лабораторной диагностики и наблюдения за возбудителем дифтерии. Автореф. дисс. д-ра мед. наук, 1993.
  5. Мазурова И.К., Комбарова С.Ю., Борисова О.Ю. и др. Мониторинг возбудителя дифтерийной инфекции. Эпидемиология и вакцинопрофилактика. 2009, 3 (46): 17-22.
  6. Максимова Н.М., Маркина С.С., Яцковский К.А. и др. Развитие эпидемического процесса дифтерии в России в условиях высокого уровня специфического иммунитета. Медицинский альманах. 2009, 2 (7): 105-108.
  7. Максимова Н.М., Маркина С.С., Яцковский К.А. и др. Дифтерия в России в 2005-2009 годах. Эпидемиология и вакцинопрофилактика. 2010, 3 (52): 31-36.
  8. Мазурова И.К., Мельников В.Г., Комбарова С.Ю. и др. Методические указания. МУ 4.2.698-98 «Лабораторная диагностика дифтерийной инфекции». М., Интэрсэн, 1998.
  9. Bolt F., Cassiday P., Tondella M. et al. Multilocus sequence typing identifies evidence for recombination and two distinct lineages of C.diphtheriae. J. Clin. Microbiol. 2010, 48 (11): 4177-4185.
  10. Grimont F., Grimont P. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann. Inst. Pasteur Microbiol. 1986, 137 В (2): 165-175.
  11. Grimont P., Grimont F., Efstraiou A. et al. International nomenclature for C.diphtheriae ri-botypes.Res. Microbiol. 2004, 153 (3): 162-166.
  12. Fafour E., Badell E., Zasada A. et al. Characterization and comparison of invasive C.diphtheriae isolates from France and Poland. J. Clin. Microbiol. 2012, 50 (1): 173-175.
  13. Maiden M., Bygraves J., Feil E. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. 1998, 95 (6): 3140-3145.
  14. Viguetti S., Pacheco L., Santos L. et al. Multilocus sequence types of invasive C. diphtheria isolated in the Rio de Janeiro urban area. Brazil. J. Epidemiol. Infect. 2011, 53:1-4.
  15. Zakikhany K., Efstratiou A. Diphtheria in Europe: current problems and new challenges. Future Microbiol. 2012, 7 (5): 595-607.
  16. Wagner K., White J., Lucenko I. et al. Diphtheria in postepidemic period, Europe, 2000-2009. J. Emerg. Infect. Dis. 2012, 18 (2): 217-225.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2013 Borisova O.Y., Mazurova I.K., Chagina I.A., Pimenova A.S., Donskikh E.E., Aleshkin V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС77-75442 от 01.04.2019 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies