Email this article COMPARATIVE CHARACTERISTIC OF BURKHOLDERIAE PSEUDOMALLEI GROUP
- Authors: Ryapis L.A1, Ilyukhin V.I2, Senina T.V2, Shubnikova E.V2, Budchenko A.A2, Kulikova A.S2
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Affiliations:
- Sechenov First Moscow State Medical University
- Research Institute of Plague Control, Volgograd, Russia
- Issue: Vol 90, No 1 (2013)
- Pages: 3-8
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13789
- ID: 13789
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Abstract
Aim . Comparative characteristic of diagnostic value of main cultural-biological characteristics of Burkholderiae pseudomallei group. Materials and methods. 59 strains of B. pseudomallei , 14 — B. mallei and 5 — B. thailandensis were used in the study. Biochemical characteristics were studied by generally accepted methods, antigenic properties were evaluated in agglutination reaction and immunoelectrophoresis, virulence was determined by Dlm for laboratory animals, antibiotic sensitivity was verified by disc-diffusion method. Results. Passaging of B. pseudomallei and B. mallei in mice results in increase of virulence, preservation of initial sensitivity to antibiotics, contraction of precipitogen specter. During therapy of experimental melioidosis in guinea pigs resistance to chemopreparations of various groups is formed. Varying degree of virulence and sensitivity to antibiotics of various B. thailandensis strains was established. Dependence of sensitivity on in vitro cultivation was not detected. Conclusion. Stability of diagnostically significant tests used for identification of Burkholderiae pseudomallei group was established. Relevance of attribute set expansion that facilitates their differentiation is justified.
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СРАВНИТЕЛЬНАЯ ХАРАКТЕРИСТИКА БУРХОЛЬДЕРИЙ ГРУППЫ PSEUDOMALLEI×
About the authors
L. A Ryapis
Sechenov First Moscow State Medical University
V. I Ilyukhin
Research Institute of Plague Control, Volgograd, Russia
T. V Senina
Research Institute of Plague Control, Volgograd, Russia
E. V Shubnikova
Research Institute of Plague Control, Volgograd, Russia
A. A Budchenko
Research Institute of Plague Control, Volgograd, Russia
A. S Kulikova
Research Institute of Plague Control, Volgograd, Russia
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