EXPRESSION OF CYTOKINES IN DENTOGINGIVAL SULCUS IN PATIENTS AFTERDENTAL IMPLANTATION AND DURING DEVELOPMENT OF PERI-IMPLANTITIS

Aim. Determination of cytokine content in various areas of dentition in patients with peri-implantitis
associated with parodontopathogenic bacteria species of I and II order. Materials and methods. 32 patients
with complications that developed in 3 months to 14 years after installation of intraosteal dental implants
were examined. Content of cytokines in various areas of dentition was determined by using solid phase
enzyme immunoassay in patients with developed peri-implantitis associated with parodontopathogenic
bacteria species of I and II order. Multiplex polymerase chain reaction was used for determination of parodontopathogenic
bacteria marker DNA. Results. Marker DNA of I order parodontopathogenic bacteria
- Aggregatibacter (Actinobacillus) actinomycetemcomitans, Tannerella forsythia (Baсteroides forsythus),
Porphyromonas gingivalis in peri-implantation tissues during implant rejection was detected with 34.4 - 75%
frequency while II order (Prevotella intermedia, Treponema denticola, Parvimonas micros (Peptostreptococcus
micros), Fusobacterium nucleatum/periodonticum etc.) - with significantly lower frequency. Total concentration
of IL-1ƒ, IL-4, IL-6, IL-8, TNFƒ, IL-17А and INFγ in contents of pathological pocket in the area of
implants and the levels of each of them were significantly higher than in the contents of parodontal pockets,
areas with stable implants and gingival fluid from areas with healthy teeth. Total interleukin content in the
contents of pathological pockets in the area of rejected implants was significantly higher than in other
studied areas. In the exudate of parodontal pockets of the preserved teeth it was 2.4 times lower, in the area
of stable implants - 4.6 times lower, and in the areas with healthy teeth - 4.8 times lower than with rejected
implants (p<0.05). Conclusion. The results of the study conducted allow to make a conclusion regard
ing reasonability of monitoring during dental implantation of parodontopathogenic microorganism strains
and local cytokine response of the host organism with the objective of rational prophylaxis and therapy of
inflammatory complications.

peri-implantitis, interleukins