Email this article FEATURES OF LEGIONELLA BIOFILM FORMATION IN ARTIFICIAL AND NATURAL WATER SYSTEMS
- Authors: Dronina Y.E1, Karpova TI1, Sadretdinova OV1, Didenko LV1, Tartakovskiy IS1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 76-80
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13739
- ID: 13739
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Abstract
Aim. Study the ability to form monospecies and associative biofilms as a characteristic of legionella
strains and features of organization of natural legionella biofilms in potentially dangerous
water systems. Materials and methods. Comparative evaluation of the ability of 28 strains of Legionella
spp. to form biofilms was determined in water according to previously developed procedure. Samples
from biofilm of industrial enterprise coolers and systems of hot water supply of public buildings
(hotels, trade centers, hospitals) were studied. Biofilms were studied by scanning and transmission
electron microscopy methods. Results. Legionella strains are divided into 3 groups by the ability
to form biofilms. L. pneumophila BLR-05 strain that has the most pronounced ability to form
monospecies biofilm and persistence in association with Pseudomonas aeruginosa was detected.
Formation of massive legionella biofilm in association with bacteria of other taxonomic groups
was detected on protective antibacterial filters in the system of hot water supply of a department
of a therapeutic prophylaxis institution in the course of 2 - 3 weeks. Legionella biofilms on the
surface of coolers resemble an aggregate of fungi, bacteria and blue-green algae enclosed into
matrix. Conclusion. The ability to form artificial monospecies and associative biofilm may be a
useful characteristic of legionella strains for evaluation of their adhesion and be used to evaluate
epidemiological significance of the isolated strains. Prevention of formation of natural legionella
biofilms in potentially dangerous water systems is necessary as an essential component of modern
strategy of legionellosis prophylaxis.
strains and features of organization of natural legionella biofilms in potentially dangerous
water systems. Materials and methods. Comparative evaluation of the ability of 28 strains of Legionella
spp. to form biofilms was determined in water according to previously developed procedure. Samples
from biofilm of industrial enterprise coolers and systems of hot water supply of public buildings
(hotels, trade centers, hospitals) were studied. Biofilms were studied by scanning and transmission
electron microscopy methods. Results. Legionella strains are divided into 3 groups by the ability
to form biofilms. L. pneumophila BLR-05 strain that has the most pronounced ability to form
monospecies biofilm and persistence in association with Pseudomonas aeruginosa was detected.
Formation of massive legionella biofilm in association with bacteria of other taxonomic groups
was detected on protective antibacterial filters in the system of hot water supply of a department
of a therapeutic prophylaxis institution in the course of 2 - 3 weeks. Legionella biofilms on the
surface of coolers resemble an aggregate of fungi, bacteria and blue-green algae enclosed into
matrix. Conclusion. The ability to form artificial monospecies and associative biofilm may be a
useful characteristic of legionella strains for evaluation of their adhesion and be used to evaluate
epidemiological significance of the isolated strains. Prevention of formation of natural legionella
biofilms in potentially dangerous water systems is necessary as an essential component of modern
strategy of legionellosis prophylaxis.
Keywords
References
- Ильина Т.С., Романова Ю.М., Гинцбург А.Л. Биопленки как способ существования бактерий в окружающей среде и организме хозяина: феномен, генетический контроль и системы регуляции их развития. Генетика. 2004, 40 (11): 1445-1456.
- Карпова Т.И., Дронина Ю.Е., Алексеева Н.В. и др. Формирование биопленок Legionella spp. в эксперименте. Журн. микробиол. 2008, 1:3-7.
- Методические указания по выявлению бактерий Legionella pneumophila в объектах окружающей среды. МУК 4.2.22-17-07. М., 2007.
- Онищенко Г.Г., Демина Ю.В., Тартаковский И.С. Современная концепция организации эпидемиологического надзора за легионеллезной инфекцией. Журн. микробиол. 2009, 5: 85-91.
- Санитарные Правила 3.1.2.2626-10. Профилактика легионеллеза.
- Темежникова Н.Д.,Тартаковский И.С. Легионеллезная инфекция. М., Меди цина, 2007.
- Тартаковский И.С., Новокшонова И.В., Cадретдинова О.В.и др. Изучение частоты и уровня контаминации Legionella pneumophila потенциально опасных водных объектов в московском регионе. Журн. микробиол. 2010, 6: 21-25.
- Atlas R.M. Legionella: from environmental habitats to disease pathology: detection and control. Environ. Microbiol. 1999, 1 (4): 283-293.
- Ito S., Karnovsky M.J. Formaldehyde/glutaraldehyde fixatives containing trinitro compounds. J. Cell. Biol. 1968, 39: 168a-169a.
- Edward S. Reynolds J.The use of lead citrate at high pH as an electron-opaque stain in electron microscopy J. Cell. Biol. 1963, 1; 17 (1): 208-212.
- Legionella and the prevention of legionellosis. WHO, 2007.
- Reisner A., Haagensen J., Schembri M. et al. Development and maturation of Escherichia coli K-12 biofilms. Mol. Мicrobiol. 2003, 48: 933-946.
- Rogues A., Boulestreau H. et al. Contribution of tap water to patient colonization with Pseudomonas aeruginosa in a medical intensive care unit. J. Hosp. Infection. 2007, 67: 72- 78.
- Van der Kooij D., Veenendal H.R., Scheffer W. Biofilm formation and multiplication of Legionella in a model warm water system with pipes of cooper, stainless steel and cross-linked polyethylene. Water. Res. 2005, 39 (13): 2789-2798.
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