Email this article MOLECULAR-GENETIC CHARACTERISTIC OF HIV-1 A AND B SUBTYPES VARIANTS ISOLATEDIN NOVOSIBIRSK REGION
- Authors: Bogachev VV1, Totmenin AV1, Baryshev PB1, Meshcheryakova Y.V1, Chernousova NY.1, Gashnikova NM1, Bogachev VV2, Totmenin AV2, Baryshev PB2, Mescheryakova Y.V3, Chernousova NY.3, Gashnikova NM2
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Affiliations:
- State Scientific Centre of Virology and Biotechnology Vector
- Novosibirsk Regional Centre of Prophylaxis andControl of AIDS and Infectious Diseases, Koltsovo, Novosibirsk region, Russia
- Issue: Vol 89, No 6 (2012)
- Pages: 45-52
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13714
- ID: 13714
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Abstract
region (NSR). Materials and methods. 268 HIV-1 variants isolated in 2007 - 2010 from blood samples of
HIV infected patients in NSR, Samara, Congo and Moscow. HIV-1 variant genotyping was performed by
analysis of 1.3 kb long pol gene nucleotide sequences. Phylogenetic analysis of nucleotide sequences was
carried out by program Mega version 4.1 by constructing phylogenetic trees by nearest neighbor method.
Nucleotide distances were calculated by Kimura method. Results. The studied HIV-1 subtype B variants form
separate phylogenetic groups with a low HIV-1 nucleotide sequence homology level combined based on
territorial principle and/or time of HIV infection in a territory but not possessing interconnection with a
specific population risk group. Subtype A HIV-1 is a fairly homogenous monophyletic group. Phylogenetic
differences during studies of HIV-1 isolated from risk group patients - injection drugs users and individuals
infected through sexual contacts were not detected. HIV variants isolated from patients infected in Moscow
and Samara generally grouped with HIV variants circulating in the European part of Russia. Conclusion. An
independent circulation of genetically separate HIV-1 subtype B groups is observed on the territory of siberian
region which is a result of multiple independent introductions of distant variants of the virus. The confirmed
limited spread of this HIV-1 genetic variant with a subsequent territorial separateness creates a possibility
of formation of genetically different virus populations. The studies of subtype A viruses performed
confirm the high level of homogeneity detected earlier in other Russia territories of HIV-1 belonging to this
genetic variant. Monophyly of subtype A HIV variants is explained by imposition of 2 factors - territorial
mobility of the population inside the country and lack of specific transmission routes for HIV-1 subtype A.
About the authors
V V Bogachev
A V Totmenin
P B Baryshev
Yu V Meshcheryakova
N Ya Chernousova
N M Gashnikova
V V Bogachev
State Scientific Centre of Virology and Biotechnology VectorState Scientific Centre of Virology and Biotechnology Vector
A V Totmenin
State Scientific Centre of Virology and Biotechnology VectorState Scientific Centre of Virology and Biotechnology Vector
P B Baryshev
State Scientific Centre of Virology and Biotechnology VectorState Scientific Centre of Virology and Biotechnology Vector
Yu V Mescheryakova
Novosibirsk Regional Centre of Prophylaxis andControl of AIDS and Infectious Diseases, Koltsovo, Novosibirsk region, RussiaNovosibirsk Regional Centre of Prophylaxis andControl of AIDS and Infectious Diseases, Koltsovo, Novosibirsk region, Russia
N Ya Chernousova
Novosibirsk Regional Centre of Prophylaxis andControl of AIDS and Infectious Diseases, Koltsovo, Novosibirsk region, RussiaNovosibirsk Regional Centre of Prophylaxis andControl of AIDS and Infectious Diseases, Koltsovo, Novosibirsk region, Russia
N M Gashnikova
State Scientific Centre of Virology and Biotechnology VectorState Scientific Centre of Virology and Biotechnology Vector
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