Email this article INTERACTION OF BIFIDOBACTERIUM BIFIDUM WITH MEMBERS OF NORMAL MICROFLORA IN HUMAN INTESTINE MICROSYMBIOCENOSIS
- Authors: Bukharin OV1, Perunova NB1, Ivanova EV1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 51-56
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13713
- ID: 13713
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Abstract
Aim. Study the influence of exometabolites of B. bifidum on biological properties of bacteria
that are the members of normoflora and their ability to interact with associative microsymbionts.
Materials and methods. Bacterial strains that are members of the normal microflora of human
intestine: B. bifidum, Lactobacillus acidophilus, Enterococcus faecium and Escherichia coli lactose
positive non-hemolytic (lac +/ hly -) were used. As opportunistic microorganisms cultures of
E. coli lactose negative hemolytic (lac -/hly +), Klebsiella pneumoniaе and Staphylococcus
aureus were used. Isolation and identification of microorganisms was performed by generally accepted
methods according to guidances. In the first series of experiments influence of B. bifidum
metabolites on biological properties of microorganisms that are members of normoflora was studied.
In the second series - the influence of bifidobacteria supernatants on interrelations of B.
bifidum, L. acidophilus and E. coli lac +/hly - with opportunistic associants. Growth properties
(GP), biofilm formation (BFF) and anti-lysozyme activity (ALA) of microorganisms was
studied photometrically. Optical density measurement were performed on ELx808 (BioTek, USA)
photometer. The data obtained were treated by nonparametric method using Mann-Whitney
criteria. Results. B. bifidum supernatant was established to stimulate in 33.3 - 66.7% of cases or
did not alter growth/reproduction, BFF and ALA of microorganisms that are characteristic for
eubiosis of intestine including bacteria of the same species that could have implications for realization
by bifidobacteria of biotope colonization resistance. Features of interaction of exometabolites
of bifidobacteria with microorganisms that are characteristic for eubiosis of human intestine consisting
in enchantment or changes of effects of the influence of normoflora members on BFF of
associants were revealed. The maximum enchantment of inhibitory effect of indigenous strains
under the influence of bifidobacteria was noted in associations E. coli lac +/hly - - E. coli lac
-/hly + as well as E. faecium - S. аureus. Conclusion. Thus, the data obtained may be used for
detection of mechanisms of functioning of normal microsymbiocenosis in human associative
symbiosis.
that are the members of normoflora and their ability to interact with associative microsymbionts.
Materials and methods. Bacterial strains that are members of the normal microflora of human
intestine: B. bifidum, Lactobacillus acidophilus, Enterococcus faecium and Escherichia coli lactose
positive non-hemolytic (lac +/ hly -) were used. As opportunistic microorganisms cultures of
E. coli lactose negative hemolytic (lac -/hly +), Klebsiella pneumoniaе and Staphylococcus
aureus were used. Isolation and identification of microorganisms was performed by generally accepted
methods according to guidances. In the first series of experiments influence of B. bifidum
metabolites on biological properties of microorganisms that are members of normoflora was studied.
In the second series - the influence of bifidobacteria supernatants on interrelations of B.
bifidum, L. acidophilus and E. coli lac +/hly - with opportunistic associants. Growth properties
(GP), biofilm formation (BFF) and anti-lysozyme activity (ALA) of microorganisms was
studied photometrically. Optical density measurement were performed on ELx808 (BioTek, USA)
photometer. The data obtained were treated by nonparametric method using Mann-Whitney
criteria. Results. B. bifidum supernatant was established to stimulate in 33.3 - 66.7% of cases or
did not alter growth/reproduction, BFF and ALA of microorganisms that are characteristic for
eubiosis of intestine including bacteria of the same species that could have implications for realization
by bifidobacteria of biotope colonization resistance. Features of interaction of exometabolites
of bifidobacteria with microorganisms that are characteristic for eubiosis of human intestine consisting
in enchantment or changes of effects of the influence of normoflora members on BFF of
associants were revealed. The maximum enchantment of inhibitory effect of indigenous strains
under the influence of bifidobacteria was noted in associations E. coli lac +/hly - - E. coli lac
-/hly + as well as E. faecium - S. аureus. Conclusion. Thus, the data obtained may be used for
detection of mechanisms of functioning of normal microsymbiocenosis in human associative
symbiosis.
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