Email this article EXPERIENCE OF APPLICATION OF MULTIPLEX QPCR FOR INTESTINE VIRAL INFECTION DIFFERENTIALDIAGNOSTICS
- Authors: Marova AA1, Oksanich AS1, Kaira AN1, Meskina ER1, Medvedeva EA1, Ivanova OE1, Lukashev AN1, Kyuregyan KK1, Kalinkina MA1, Egorova OV1, Zverev VV1, Fayzuloev EB1, Marova AA2, Oksanich AS2, Kaira AN2, Meskina ER3, Medvedeva EA3, Ivanova OE4, Lukashev AN4, Kyuregyan KK4, Kalinkina MA5, Egorova OV5, Zverev VV2, Faizuloev EB2
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Affiliations:
- Mechnikov Research Institute of Vaccines and Sera
- Vladimirsky Moscow Regional Scientific Research ClinicalInstitute
- Chumakov Research Institute of Poliomyelitis and Viral Encephalitis
- Innovation-technological CentreBiologically Active Compounds and Their Application, Moscow, Russia
- Issue: Vol 89, No 6 (2012)
- Pages: 39-45
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13711
- ID: 13711
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Abstract
fluorescence detection in real time mode (qPCR) methods for differential detection of 11 groups of intestine
viruses (adenoviruses, enteroviruses, polioviruses, hepatitis A and E viruses, group A and C rotaviruses, orthoreoviruses,
noroviruses, sapoviruses and astroviruses) in various biological samples. Materials and methods.
Panels of virus isolates and clinical samples characterized by reference methods were used to evaluate sensitivity
of detection of various intestine viruses. Nucleic acids were isolated from study samples and multiplex
RT and qPCR were carried out. Results. Sensitivity of laboratory reagent kit (LRK) when compared with
results obtained from reference methods was 100% for rotavirus A, adenovirus, enterovirus and norovirus,
88.9% for hepatitis E virus and 92.3% for hepatitis A virus, and diagnostic specificity - 99.4%. During
analysis of 697 clinical samples from patients with acute intestine infection symptoms nucleic acids of various
intestine viruses were isolated in 71.7%. Conclusion. Multiplex qRT-PCR was shown as an effective method
of etiologic diagnostics of an intestine viral infection. Use of LRK was demonstrated to establish etiology of
intestine diseases in 63 - 72% and in children with watery diarrhea - in approximately 90% of cases.
About the authors
A A Marova
A S Oksanich
A N Kaira
E R Meskina
E A Medvedeva
O E Ivanova
A N Lukashev
K K Kyuregyan
M A Kalinkina
O V Egorova
V V Zverev
E B Fayzuloev
A A Marova
Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera
A S Oksanich
Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera
A N Kaira
Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera
E R Meskina
Vladimirsky Moscow Regional Scientific Research ClinicalInstituteVladimirsky Moscow Regional Scientific Research ClinicalInstitute
E A Medvedeva
Vladimirsky Moscow Regional Scientific Research ClinicalInstituteVladimirsky Moscow Regional Scientific Research ClinicalInstitute
O E Ivanova
Chumakov Research Institute of Poliomyelitis and Viral EncephalitisChumakov Research Institute of Poliomyelitis and Viral Encephalitis
A N Lukashev
Chumakov Research Institute of Poliomyelitis and Viral EncephalitisChumakov Research Institute of Poliomyelitis and Viral Encephalitis
K K Kyuregyan
Chumakov Research Institute of Poliomyelitis and Viral EncephalitisChumakov Research Institute of Poliomyelitis and Viral Encephalitis
M A Kalinkina
Innovation-technological CentreBiologically Active Compounds and Their Application, Moscow, RussiaInnovation-technological CentreBiologically Active Compounds and Their Application, Moscow, Russia
O V Egorova
Innovation-technological CentreBiologically Active Compounds and Their Application, Moscow, RussiaInnovation-technological CentreBiologically Active Compounds and Their Application, Moscow, Russia
V V Zverev
Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera
E B Faizuloev
Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and Sera
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