Email this article VIBRIO CHOLERAE STRAINS CERTIFICATION
- Authors: Kislichkina AA1, Stepanshina VN1, Nizova AV1, Mayskaya NV1, Mukhina TN1, Mironova RI1, Shemyakin IG1, Kislichkina AA2, Stepanshina VN2, Nizova AV2, Mayskaya NV2, Mukhina TN2, Mironova RI2, Shemyakin IG2
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Affiliations:
- State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
- Issue: Vol 89, No 6 (2012)
- Pages: 22-26
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13700
- ID: 13700
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Abstract
cultures SCPM - Obolensk. Materials and methods. 50 V. cholerae strains were studied. Real-time PCR with
primers to genes ctxA, ctxB, ace, zot, tсpА, toxR, hlyA, rtxC, rfbO1, ompU, ompW was used. Results.
Membership of the studied strains in V. cholerae species was confirmed by molecular-biological methods. 46
strains belong to O1 serogroup, 42 of those - El Tor toxigenic, having all the virulence genes and 4 nontoxigenic
strains. A strain had ace, zot, tсpA, toxR, rtxC, hlyA, ompU genes. 2 strains had ace, toxR, rtxC,
hlyA genes, a strain had only toxR gene which is a global regulatory gene. 2 of the 4 serogroup O1 strains were
non-toxigenic and had all the virulence genes (ctxA, ctxB, ace, zot, tсpA, toxR, rtxC, hlyA, ompU). 1 nontoxigenic
strain has ace, zot, toxR, hlyA, ompU genes, and the other - toxR, hlyA genes. Conclusion. Genome
certificates of all the V. cholerae strains stored in State collection of pathogenic microorganisms and cell
cultures SCPM - Obolensk were created. Markers of epidemic threat - ctxA, ctxB, tcpA, toxR and additional
virulence genes were determined.
About the authors
A A Kislichkina
V N Stepanshina
A V Nizova
N V Mayskaya
T N Mukhina
R I Mironova
I G Shemyakin
A A Kislichkina
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
V N Stepanshina
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
A V Nizova
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
N V Mayskaya
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
T N Mukhina
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
R I Mironova
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
I G Shemyakin
State Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, RussiaState Scientific Centre of Applied Microbiology and Biotechnology, Obolensk, Moscow region, Russia
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