Email this article PATHOGENICITY FACTORS OF ENTEROCOCCI OF HUMAN INTESTINE MICROFLORA
- Authors: Valyshev AV1, Gertsen NV1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 41-44
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2012
- URL: https://microbiol.crie.ru/jour/article/view/13697
- ID: 13697
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Abstract
Aim. Characteristic of pathogenicity factors of enterococci isolated from human feces. Materials
and methods. Production of hemolysin, gelatinase and DNase was determined in 161 enterococci
cultures. Results. Hemolytic activity detected in 14.92.8% of the studied cultures was the most
prevalent characteristic; 22 of 24 hemolytic strains belonged to E. faecalis species. Human erythrocyte
lysis was also caused by E. faecium and E. durans cultures (1 strain each). Other pathogenicity
factors were detected solely in E. faecalis species members. Enterococci proteolytic activity
associated with gelatinase enzyme production manifested on various substrates - both gelatin and
milk. This property was detected in 7.52.1% cultures. Deoxyribonuclease was detected in 1
(1.20.9%) E. faecalis strain. A number of E. faecalis cultures possessing hemolytic activity additionally
hydrolyzed gelatin (22.78.9% strains) and DNA (4.54.4% isolates). Conclusion.
Though pathogenicity factors occur in enterococci of intestine microflora relatively rarely, separate
cultures with expression of 2 or more pathogenicity factors may be essential in the development
of endogenous infections especially in immune compromised patients.
and methods. Production of hemolysin, gelatinase and DNase was determined in 161 enterococci
cultures. Results. Hemolytic activity detected in 14.92.8% of the studied cultures was the most
prevalent characteristic; 22 of 24 hemolytic strains belonged to E. faecalis species. Human erythrocyte
lysis was also caused by E. faecium and E. durans cultures (1 strain each). Other pathogenicity
factors were detected solely in E. faecalis species members. Enterococci proteolytic activity
associated with gelatinase enzyme production manifested on various substrates - both gelatin and
milk. This property was detected in 7.52.1% cultures. Deoxyribonuclease was detected in 1
(1.20.9%) E. faecalis strain. A number of E. faecalis cultures possessing hemolytic activity additionally
hydrolyzed gelatin (22.78.9% strains) and DNA (4.54.4% isolates). Conclusion.
Though pathogenicity factors occur in enterococci of intestine microflora relatively rarely, separate
cultures with expression of 2 or more pathogenicity factors may be essential in the development
of endogenous infections especially in immune compromised patients.
References
- Бухарин О. В., Валышев А. В. Биология и экология энтерококков. Екатеринбург, УрО РАН, 2011.
- Baldassarri L., Creti R., Arciola C.R. et al. Analysis of virulence factors in cases of enterococcal endocarditis. Clin. Microbiol. Infect. 2004, 10 (11): 1006-1008.
- Elsner H.A., Sobottka I., Mack D. et al. Virulence factors of Enterococcus faecalis and Enterococcus faecium blood culture isolates. Eur. J. Clin. Microbiol. Infect. Dis. 2000, 19 (1): 39-42.
- Kayaoglu G., Ørstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit. Rev. Oral Biol. Med. 2004, 15 (5): 308-320.
- Sedgley C.M., Molander A., Flannagan S.E. et al. Virulence, phenotype and genotype characteristics of endodontic Enterococcus spp. Oral Microbiol. Immunol. 2005, 20 (1): 10-19.
- Semedo T., Santos M.A., Lopes M.F. et al. Virulence factors in food, clinical and reference enterococci: A common trait in the genus? Syst. Appl. Microbiol. 2003, 26 (1): 13-22.
- Willems R.J., Hanage W.P., Bessen D.E., Feil E.J. Population biology of Gram-positive pathogens: high-risk clones for dissemination of antibiotic resistance. FEMS Microbiol. Rev. 2011, 35 (5): 872-900.
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