Email this article CONTRIBUTION OF L,D-CARBOXYPEPTIDASES IN VIRULENCE OF FACULTATIVE INTRACELLULAR PATHOGENIC BACTERIA LISTERIA MONOCYTOGENES
- Authors: Yurov DS1, Varfolomeev AF1, Tukhvatulin AI1, Logunov DY.1, Naroditskiy BS1, Ermolaeva SA1
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Affiliations:
- Issue: Vol 89, No 4 (2012)
- Pages: 15-20
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13676
- ID: 13676
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Abstract
Aim. Evaluate influence of mutation of Listeria monocytogenes genes coding murein-tetrapeptide
L,D-carboxypeptidase Lmo0028 and Lmo1638 on dynamics of infectious process and interaction
of purified muropeptides with NOD1 receptor. Materials and methods. Wild type EGDe strain and
recombinant strains GIMins1638 и GIMins0028 obtained on its basis by site-specific mutagenesis
were used. Infectious process dynamics was studied on the model of intravenous infection of
BALB/c mice. Ligand-receptor interaction activity of muropeptides isolated from recombinant
and parent strains were assayed on HEK293-hNOD1 cell line expressing NOD1 receptor and
containing in their genome -galactosidase reporter gene under the control of NF-kB dependent
promoter expression. Results. Lack of Lmo0028 decelerates reproduction of listerias in animal
liver starting from 24 hours and at later terms after the infection whereas lack of Lmo1638 leads
to increase of microbial load 6 and 24 hours after the infection with no influence on further infection.
Differences in activation of NOD1 receptor by muropeptides isolated from recombinant and
parent strains were not detected. Conclusion. Despite high homology murein-tetrapeptide L,Dcarboxypeptidase
Lmo0028 and Lmo1638 make a different contribution to the development of
infectious process caused by L. monocytogenes in BALB/c line mice. Lack of differences in NOD1
receptor activation may be associated with compensation of enzymatic functions in strains with
mutation in each of the genes owing to the presence of homologous protein.
L,D-carboxypeptidase Lmo0028 and Lmo1638 on dynamics of infectious process and interaction
of purified muropeptides with NOD1 receptor. Materials and methods. Wild type EGDe strain and
recombinant strains GIMins1638 и GIMins0028 obtained on its basis by site-specific mutagenesis
were used. Infectious process dynamics was studied on the model of intravenous infection of
BALB/c mice. Ligand-receptor interaction activity of muropeptides isolated from recombinant
and parent strains were assayed on HEK293-hNOD1 cell line expressing NOD1 receptor and
containing in their genome -galactosidase reporter gene under the control of NF-kB dependent
promoter expression. Results. Lack of Lmo0028 decelerates reproduction of listerias in animal
liver starting from 24 hours and at later terms after the infection whereas lack of Lmo1638 leads
to increase of microbial load 6 and 24 hours after the infection with no influence on further infection.
Differences in activation of NOD1 receptor by muropeptides isolated from recombinant and
parent strains were not detected. Conclusion. Despite high homology murein-tetrapeptide L,Dcarboxypeptidase
Lmo0028 and Lmo1638 make a different contribution to the development of
infectious process caused by L. monocytogenes in BALB/c line mice. Lack of differences in NOD1
receptor activation may be associated with compensation of enzymatic functions in strains with
mutation in each of the genes owing to the presence of homologous protein.
Keywords
About the authors
D S Yurov
A F Varfolomeev
A I Tukhvatulin
D Yu Logunov
B S Naroditskiy
S A Ermolaeva
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