DETECTION OF RUBELLA VIRUS RNA IN CLINICALMATERIAL BY REAL TIME POLYMERASECHAIN REACTION METHOD

Aim. Development of a reagent kit for detection
of rubella virus RNA in clinical material by
PCR-RT. Materials and methods. During development
and determination of analytical specificity
and sensitivity DNA and RNA of 33
different microorganisms including 4 rubella
strains were used. Comparison of analytical
sensitivity of virological and molecular-biological
methods was performed by using rubella
virus strains Wistar RA 27/3, M-33, «Orlov»,
Judith. Evaluation of diagnostic informativity
of rubella virus RNA isolation in various clinical
material by PCR-RT method was performed in
comparison with determination of virus specific
serum antibodies by enzyme immunoassay.
Results. A reagent kit for the detection of rubella
virus RNA in clinical material by PCR-RT
was developed. Analytical specificity was 100%,
analytical sensitivity - 400 virus RNA copies
per ml. Analytical sensitivity of the developed
technique exceeds analytical sensitivity of the
Vero E6 cell culture infection method in studies
of rubella virus strains Wistar RA 27/3 and
«Orlov» by 1 lg and 3 lg, and for M-33 and Judith
strains is analogous. Diagnostic specificity is
100%. Diagnostic specificity for testing samples
obtained within 5 days of rash onset: for peripheral
blood sera - 20.9%, saliva - 92.5%, nasopharyngeal
swabs - 70.1%, saliva and nasopharyngeal
swabs - 97%. Positive and
negative predictive values of the results were
shown depending on the type of clinical material
tested. Conclusion. Application of reagent
kit will allow to increase rubella diagnostics effectiveness
at the early stages of infectious process
development, timely and qualitatively perform
differential diagnostics of exanthema
diseases, support tactics of anti-epidemic regime.

rubella, rubella virus RNA, real time PCR, diagnostics