Email this article RASPROSTRANENIE GLYuKOZILTRANSFERAZY Lgt SREDI ShTAMMOV LEGIONELLAPNEUMOPHILA, VYDELENNYKh IZ RAZLIChNYKh ISTOChNIKOV
- Authors: Sadretdinova OV1, Lyuk K1, Karpova TI1, Belyy Y.F1, Tartakovskiy IS1
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- Issue: Vol 89, No 3 (2012)
- Pages: 8-12
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/13662
- ID: 13662
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Abstract
Aim. Determine various members of Lgt glucosyl transferase family among microorganisms of
Legionellaceae genus from museum collection and legionellae strains recently isolated in the
Russian Federation and Germany. Materials and methods. Presence of 3 types of glucosyl transferase
were determined in 73 strains of L. pneumophila and Legionella spp. Glucosyl transferase activity
of 3 types (Lgt1, Lgt2 и Lgt3) was determined by western blotting and PCR method. Results. Lgt1
and Lgt3 were detected only in members of L. pneumophila independently of isolation source and
were absent in Legionella spp. strains. Lgt2 is absent in Legionella spp. strains and is detected in not
all the L. pneumophila strains. Comparative analysis of detection frequency of Lgt2 in clinical
strains and L. pneumophila strains isolated from the environment showed that the protein is detected
in clinical strains more frequently (46%) compared with strains from the environment (23%).
Conclusion. Lgt1 and Lgt3 as species specific markers could be used for practical purposes for
identification of L. pneumophila strains. High frequency of Lgt2 isolation in clinical strains of L.
pneumophila isolated from lung tissue in lethal cases of legionellosis compared with strains isolated
from the environment requires a more detailed study of functional activity and substrate
specificity of the glucosyl transferase.
Legionellaceae genus from museum collection and legionellae strains recently isolated in the
Russian Federation and Germany. Materials and methods. Presence of 3 types of glucosyl transferase
were determined in 73 strains of L. pneumophila and Legionella spp. Glucosyl transferase activity
of 3 types (Lgt1, Lgt2 и Lgt3) was determined by western blotting and PCR method. Results. Lgt1
and Lgt3 were detected only in members of L. pneumophila independently of isolation source and
were absent in Legionella spp. strains. Lgt2 is absent in Legionella spp. strains and is detected in not
all the L. pneumophila strains. Comparative analysis of detection frequency of Lgt2 in clinical
strains and L. pneumophila strains isolated from the environment showed that the protein is detected
in clinical strains more frequently (46%) compared with strains from the environment (23%).
Conclusion. Lgt1 and Lgt3 as species specific markers could be used for practical purposes for
identification of L. pneumophila strains. High frequency of Lgt2 isolation in clinical strains of L.
pneumophila isolated from lung tissue in lethal cases of legionellosis compared with strains isolated
from the environment requires a more detailed study of functional activity and substrate
specificity of the glucosyl transferase.
References
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