ANTAGONISTIC ACTIVITY OF LACTOBACILLIOF THE COLON

Aim. Study of antagonistic activity of lactobacilli
of the colon against members of its autochthonous
bacterial flora and agents of some
acute infectious and chronic diseases of the
gastrointestinal tract. Materials and methods.
Antagonistic activity of 19 lactobacilli cultures
against 28 cultures of bacteria belonging to
various groups and fungi was evaluated within
the framework of specially developed two-stage
cultivation technique in the conditions of a
combined system. The results of the study were
evaluated according to a semi-quantitative scale
that allows to put one or the other value of the
zone of growth delay of the studied strain culture
in compliance with the one or the other (low,
moderate, high) level of antagonistic activity of
the lactobacillus culture. Results. Lactobacilli
of the colon showed selective antagonistic activity
against pathogenic enterobacteriae: pronounced
against Salmonella enterica Serovar
Enteritidis, Shigella flexneri 2b, Yersinia spp., and
trace against Salmonella enterica Serovar
Typhimurium. The level of antagonistic activity
of lactobacilli against a wide range of members
of autochthonous bacterial flora varied in a wide
range, without revealing connection neither to
its belonging to species, nor to its population
level, nor to the belonging to group of the antagonistic
effect objects. On the other hand a
connection was traced with belonging to a certain
microbiota: being quite active against
members of its own microbiota, lactobacilli
often showed significantly lower level of antagonistic
activity against cultures with the same
species name isolated from other microbiota.
Conclusion. In light of the results obtained,
level of lactobacilli population may hardly be
viewed as the only criteria of their full participation
in the process of stabilizing microecological
welfare of the colon, that allows to make a
complete representation of the level of dysbiotic
disorder in the mentioned biotope. With in
the framework of rational bacteriological diagnostics
of the level of dysbiotic disorders in the
colon, evaluation of population level of lactobacilli
should be evaluated along with the degree
of their antagonistic activity against other components
of the same microbiota.

interaction of microorganisms, antagonistic activity, lactobacilli, colon microflora, autochthonous microflora, pathogenic microflora