TAXONOMIC CHARACTERISTICS AND MIXEDCOMMUNITIES OF WOUND INFECTIONAGENTS IN PATIENTS OF REANIMATION ANDSURGICAL DEPARTMENTS OF A HOSPITAL

Aim. Study of taxonomical structure of wound
infection agents, prevalence of mixes, and detection
of character of their possible connection
with the results of various microorganisms
population interaction in septic wounds.
Materials and methods. A microbiological study
of material from patients with wound infection
(WI), 582 of those were cured in reanimation
and intensive therapy departments (RITD;
group 1) and 1455 - in surgical departments
(SD; group 2), was performed. Taxonomic
membership and ability to coexist was determined
in 4129 microorganisms strains. Etiological
role of the agents was evaluated by using
values of consistency rate (CR). Species that
were present in more than 50% of samples were
considered consistent, in 25 to 50% - additional,
and in less than 25% - random.
Frequency rates (FR) were also determined,
that is the fraction of a certain species (genus)
of the microorganism (in %) from all the isolated
cultures that correspond to 100%. For the
determination of the significance of individual
species of the agent in the structure of mixed
microorganism populations, FR - their fraction
(%) in mixed population from the number of
strains of this species that correspond to 100%
- was calculated. Results. A significant part of
the microorganisms strains, more frequently in
reanimation department (65.5%), caused wound
suppuration in populations mixed with other
species of the agents. In reanimation and surgical
departments consistent species of wound
infection agents were not detected. A leading
etiological role of Staphylococcus aureus (FR
19.2% and 23.9%) was determined, and FR of
S. aureus strains in mixes was 64.6% in RITD
and 46.8% in SD. The parameters of other agents
of WI in the comparison groups were similar.
However FR among mixes in RITD were sig-
nificantly higher for streptococci that do not
belong to S. pyogenes species (72,5%), and also
nonfermentative microorganisms (67,2%), and
in SD - in Klebsiella pneumoniae mixes. For
agents of wound infection especially in RITD,
low species diversity was characteristic and the
number of mixes variants is significantly higher.
In RITD mixed infections develop more frequently,
and the ecological community of microorganisms
reaches higher values than in SD.
Conclusion. During the analysis of microbiological
data in RITD and SD general patterns and
specific features of taxonomical structure,
prevalence of mixed populations and character
of their ecological community in wound infection
was determined.

wound infection agents, mixed microorganisms communities, ecological community of agents