IMMUNOGENIC PROPERTIES AND MOLECULAR-GENETIC FEATURES OF NATIVE AND INACTIVATED CHLAMYDIA TRACHOMATIS MT-2A SEROVARIANT D STRAIN

Aim. Study of antibody genesis after introduction of native and inactivated Chlamydia trachomatis МТ-2А strain into Balb/c mice and analysis of molecular-genetic features of omp A gene that is responsible for its immunogenicity. Materials and methods. Chlamydia infection was modeled in Balb/c mice by using human isolated C.trachomatis serovariant D. Results. After the introduction of this strain, 5 days after the infection of mice A, M and G class antibodies including G class antibodies against chlamydia heat shock protein are induced. Studies of molecular-genetic features of the isolated C.trachomatis МТ-2А strain showed presence of 2 nucleotide mutations in ompA gene. A synonymous substitution of cytosine to thymine in position 574 (CD) that does not result in amino acid substitution in the protein was registered. A non-synonymous substitution of thymine to guanine was present in position 843 (VDIII), that is most probably one of the main reasons of the registered higher immunogenicity of C.trachomatis МТ-2А serovariant D strain. Conclusion. Further studies of C.trachomatis МТ-2А serovariant D strain are promising, but do not exclude search of other immunogenic strains for the generation of agents for specific prophylaxis and immunotherapy of chlamydiosis.

Chlamydia trachomatis MT-2A serovariant D, chlamydiosis, native and inactivated strains