MHC-MULTIMERS AND THEIR APPLICATION IN STUDIES OF ANTIVIRAL IMMUNE RESPONSE


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Abstract

Application of main histocompatibility complex tetrames (MHC-tetramers) for antigen specific T-cells detection and analysis coupled with flow cytometry opened new opportunities for T-cell response analysis. MHC-multimers allow the de- tection of T-cells against viral, cancer and vaccine antigens with exceptional sensitivity and specificity. This approach has become the «gold standard» for quantative analysis of T-cell immune response. Certain aspects of analysis using MHC-tetramer are examined, and importance of this approach in T-cell response efficacy evaluation in anti-HIV vaccine trials as well as in HIV positive patients are discussed.

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MHC-МУЛЬТИМЕРЫ И ИХ ПРИМЕНЕНИЕ В ИЗУЧЕНИИ ПРОТИВОВИРУСНОГО ИММУННОГО ОТВЕТА
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About the authors

L. I Karpenko

State Scientific Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk region

L. V Mechetina

Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia

A. Yu Reguzova

State Scientific Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk region

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Copyright (c) 2011 Karpenko L.I., Mechetina L.V., Reguzova A.Y.

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