CHARACTERISTICS OF VIBRIO CHOLERAE nonO1/nonO139 SEROGROUP STRAINS THAT CAUSED DISEASES IN POPULATION OF ROSTOV REGION


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Abstract

Aim. Genotype characteristic and determination of serological properties of Vibrio cholerae nonО1/nonО139 strains that caused diseases in population of Rostov region from 2000 to 2009. Materials and methods. 15 clinical strains of V.cholerae nonO1/nonO139 were studied. Serotyping was performed by using a kit of monospecific typing sera against serogroup O2-O84 cholera vibrios obtained from Rostov Research Institute for Plague Control, PCR and VNTR-genotyping - by using specific primers described in scientific publications and constructed by us. Results. Serologic features of strains are very diverse and strains contain various combination of pathogenicity factor genes that seem to be interchangeable. Similar pattern was observed for VNTR-genotyping. Distribution of the examined strains by VNTR-genotyping did not correlate with either PCR-genotyping data or serotyping, or place and time of isolation. Conclusion. The data obtained indicates a lack of general source of human infection even in the same location and time period. On the other hand, serological and genotypic features of V.cholerae nonO1/nonO139 may undergo changes in the process of staying in the macro organism or environment due to high plasticity of their genome.

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About the authors

V. D Kruglikov

Research Institute for Plague Control, Rostov-on-Don, Russia

E. V Monakhova

Research Institute for Plague Control, Rostov-on-Don, Russia

I. V Arkhangelskaya

Research Institute for Plague Control, Rostov-on-Don, Russia

A. S Vodopyanov

Research Institute for Plague Control, Rostov-on-Don, Russia

S. O Vodopyanov

Research Institute for Plague Control, Rostov-on-Don, Russia

E. P Avdeeva

Research Institute for Plague Control, Rostov-on-Don, Russia

N. V Bozhko

Research Institute for Plague Control, Rostov-on-Don, Russia

L. M Smolikova

Research Institute for Plague Control, Rostov-on-Don, Russia

References

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Copyright (c) 2011 Kruglikov V.D., Monakhova E.V., Arkhangelskaya I.V., Vodopyanov A.S., Vodopyanov S.O., Avdeeva E.P., Bozhko N.V., Smolikova L.M.

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