REVEALING THE GENETIC DETERMINANTS OF Pks-PATHOGENICITY ISLAND IN CLINICAL STRAINS OF ENTEROBACTERIA


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Abstract

Aim. Detection by PCR the frequency of clbB, clbN, clbA и clbQ genes of Рks-pathogenicity island in clinical strains of enterobacteria. Materials and methods. 112 strains various genera and species of enterobacteria, including 16 museum and 96 clinical are investigated. Isolated strains represents Escherichia species (n=68), Klebsiella (n=16), Enterobacter (n=9), Serratia (n=7) and others minor species of Enterobacteriaceae family (n=12). Fifty nine strains isolated from urine of urinary tract infection, 26 isolates from intestines of patients with dysbiosis and 11 - from children with complications after a liver transplantation. A total bacterial isolates were screened by multiplex PCR for the presence of clbB, clbN, clbA and clbQ genes. Results. Among 41 uropathogenic E.coli it is revealed 15 (36,6%) Pks-positive strains carring all of clbB, clbN, clbA и clbQ genes, that composed 27,1% from total number of the enterobacteria, isolates from urine. Among 44 clinical isolates of various species of enterobacteria only one Pks-positive strain K.рneumoniae was revealed. Strains enterobacteria, isolated at pyoinflammatory complications after liver transplantation (n=11) and isolates from intestinal tract in dysbiosis (n=26), were Pks-negative. Conclusion. The clbB, clbN, clbA и clbQ genes of the Pks-island which have been detected in 36,6% E.coli urological strains are markers of pathogenicity of clinical isolates of extraintestinal origin and advisable of their detection by PCR.

About the authors

S. V Fialkina

V. M Bondarenko

Yu. L Naboka

Rostov State Medical University, Rostov-on-Don, Russia

E. A Miroshnichenko

Rostov State Medical University, Rostov-on-Don, Russia

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Copyright (c) 2011 Fialkina S.V., Bondarenko V.M., Naboka Y.L., Miroshnichenko E.A.

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