ENZYME IMMUNOASSAY OF MASKED COMPLEMENT COMPONENT C4 DEFICIENCY IN PATIENTS WITH UROGENITAL CHLAMYDIA INFECTION

Aim. Development of new method of C4B isotype functional activity evaluation in enzyme immunoassay by using pharmaceutical preparation derinat as a classical pathway complement activator and its use for blood sera isotyping in confirmed urogenital tract chlamydia infection. Materials and methods. Enzyme immunoassay was used to detect C4A and C4B isotype functional deficiency in blood sera of patients. Chlamydia etiology urogenital infection diagnosis was based on results of standard clinical-instrumental examination methods: vaginal clinical smear analysis, scrape sample light microscopy with consequent treatment by fluorescent monoclonal antibodies against Chlamydia trachomatis and PCR. Results. In acute form of the disease C4A deficiency frequency of occurrence was 0.36, and C4B deficiency — 0.55. In chronic form of the disease deficiency frequency of occurrence was 0.38 for both isotypes. In the group of healthy people isotype deficiency was 0.08 and 0.25, respectively. Conclusion . Innate masked C4 deficiency interfere with the normal immune defense of organism against chlamydia infection, and antigen carbohydrate pathogenicity may possibly be more significant for the development of immune response to which C4B isotype activity is necessary.

Chlamydia trachomatis, complement system, deficiency, C4B, C4A isotypes, functional activity evaluation, enzyme immunoassay, Chlamydia trachomatis, urogenital chlamydia infection