M-29 HUMAN DIPLOID CELLS LINE — A SUBSTRATE FOR THE PRODUCTION OF ANTIVIRAL VACCINES


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Abstract

Aim. Study of morphologic and karyologic characteristics of 5 russian human diploid cell lines (HDC). Materials and methods. 5 HDC lines and HDC strain MRC-5 were studied; RK-13 and Vero continuous cell lines were used; viruses: rubella (RA 27/3), measles (L-16), epidemic parotitis (L- 3). Cytogenetic analysis of HDC was performed by using DAPI differential staining method. Results. M-29 line has characteristics that are similar to those of MRC-5 diploid cell strain. M-29 cell culture is not contaminated with foreign viruses, mycoplasmata, does not have oncogenic potency. Conclusion. M-29 line has high virus-productive properties for accumulation of measles, rubella and epidemic parotitis vaccine viruses and may be recommended as a substrate for the production of antiviral vaccines.

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ЛИНИЯ ДИПЛОИДНЫХ КЛЕТОК ЧЕЛОВЕКА М-29 — СУБСТРАТ ДЛЯ ПРОИЗВОДСТВА ПРОТИВОВИРУСНЫХ ВАКЦИН
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About the authors

T. K Borisova

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

V. V Zverev

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

L. L Mironova

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

O. I Konyushko

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

Yu. I Zabiyaka

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

N. R Shukhmina

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

V. I Vaschenko

Mechnikov Research Institute of Vaccines and Sera, Moscow; Chumakov Research Institute of Poliomyelitis and Viral Encephalites, Moscow region, Russia

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Copyright (c) 2011 Borisova T.K., Zverev V.V., Mironova L.L., Konyushko O.I., Zabiyaka Y.I., Shukhmina N.R., Vaschenko V.I.

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