DEVELOPMENT AND CHARACTERIZATION OF HELICOBACTER PYLORI CagA RECOMBINANT FRAGMENTS


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Abstract

Aim. The goal of the work was to produce, purify and characterize recombinant fragments of Helicobacter pylori CagA protein. Materials and methods. The methods of molecular cloning, recombinant protein expression in Escherichia coli , affinity chromatography, gel electrophoresis and western-blotting as well as several in silico algorithms of nucleotide and aminoacid sequence analysis were used. Results. Four N-terminal His6-tagged recombinant fragments of CagA protein were produced. Protein rCagAfr.1 (65 kDa) represents the most conserved N-terminal part of the cytotoxin. Fragment rCagAfr.2 (44 kDa) corresponds to the central conserved region of CagA whereas rCagAfr.3 (39 kDa) represents the highly variable C-terminal part of CagA. Finally, the protein rCagAfr.4 (75 kDa) incorporates the sequences of rCagAfr.2 and rCagAfr.3. In silico analysis of fragments’ sequences allowed to suppose that rCagAfr.2 and rCagAfr.4 are highly immunogenic proteins. By means of chromatographic purification, high levels of purity (up to 97%) and yield (about 15 mg per L of culture) of recombinant proteins were achieved. Conclusion. Use of recombinant proteins technology allowed to solve the problem of producing the CagA pure protein of H.pylori , which open new perspectives for the development of immunodiagnostic assays for detection of CagA protein or antibodies to this cytotoxin.

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ПОЛУЧЕНИЕ И ХАРАКТЕРИСТИКА РЕКОМБИНАНТНЫХ ФРАГМЕНТОВ БЕЛКА CagA HELICOBACTER PYLORI
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About the authors

A. V Klimovich

Saint-Petersburg State University

M. P Samoylovich

Russian Research Center for Radiology and Surgical Technologies

A. N Suvorov

Institute of Experimental Medicine, Saint-Petersburg, Russia

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Copyright (c) 2010 Klimovich A.V., Samoylovich M.P., Suvorov A.N.

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